Invitrogen

FZD6 Polyclonal Antibody

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FZD6 Antibody (PA5-87953) in WB

Western blot analysis of FZD6 in extracts of various cell line. Samples were incubated with FZD6 Polyclonal antibody (Product # PA5-87953) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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产品信息

PA5-87953

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 19-201 of human FZD6 (NP_0034972)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.13 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2804535

产品详细信息

Immunogen sequence: HSLFTCEPIT VPRCMKMAYN MTFFPNLMGH YDQSIAAVEM EHFLPLANLE CSPNIETFLC KAFVPTCIEQ IHVVPPCRKL CEKVYSDCKK LIDTFGIRWP EELECDRLQY CDETVPVTFD PHTEFLGPQK KTEQVQRDIG FWCPRHLKTS GGQGYKFLGI DQCAPPCPNM YFKSDELEFA KSF; Positive Samples: mouse kidney, rat kidney; Cellular Location: Apical cell membrane, Cell membrane, Cell surface, Cytoplasmic vesicle membrane, Membrane, Multi-pass membrane protein

靶标信息

The exact function of the protein encoded by this gene is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CircSmg5 stimulates the osteogenic differentiation of bone marrow mesenchymal stem cells by targeting the miR-194-5p/Fzd6 axis and beta-catenin signaling.

Environmental toxicology

Lu Y,Liu YK,Wan FY,Shi S,Tao R

PA5-87953 was used in Immunohistochemistry (Paraffin), Western Blot to explore the function and regulatory mechanism of circSmg5 in BMSC osteogenic differentiation.

Tue Mar 01 00:00:00 UTC 2022

GATA4 regulates mesenchymal stem cells via direct transcriptional regulation of the WNT signalosome.

Bone

Khalid AB,Pence J,Suthon S,Lin J,Miranda-Carboni GA,Krum SA

PA5-87953 was used in Immunocytochemistry to suggest that WNT signalosome activity in MSCs requires Gata4 and Wnt10b expression for lineage specification.