Invitrogen

FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), APC, eBioscience™

FceR1 alpha Antibody in Flow Cytometry (Flow)

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FceR1 alpha Antibody (17-5899-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human IgE FITC (Product # 11-6986-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human Fc epsilon Receptor I alpha (FceR1) APC (right). Cells in the lymphocyte gate were used for analysis (note: basophils reside in FCS/SSC position of lymphocytes).

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产品信息

17-5899-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

AER-37 (CRA1)

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10671394

产品详细信息

Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported: The AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AER-37 (CRA1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera.

Diagnostics (Basel, Switzerland)

Prakash PS,Barwary NJS,Weber MHW,Wan D,Conejeros I,Pereira Moreira B,Alharbi WS,van Hellemond JJ,Akinwale J,Falcone FH

Published figure using FceR1 alpha monoclonal antibody (Product # 17-5899-42) in Flow Cytometry

Thu Aug 25 00:00:00 UTC 2022

Phenotypic and Histological Distribution Analysis Identify Mast Cell Heterogeneity in Non-Small Cell Lung Cancer.

Cancers

Leveque E,Rouch A,Syrykh C,Mazières J,Brouchet L,Valitutti S,Espinosa E,Lafouresse F

17-5899-42 was used in flow cytometry to reveal that TAMCs constitute a heterogeneous population and indicates that MC subsets should be considered for patients' stratification and management in future research.

Wed Mar 09 00:00:00 UTC 2022

Directed evolution of and structural insights into antibody-mediated disruption of a stable receptor-ligand complex.

Nature communications

Pennington LF,Gasser P,Kleinboelting S,Zhang C,Skiniotis G,Eggel A,Jardetzky TS

17-5899-42 was used in flow cytometry to show how kinetically active disruptive antibodies can be used to accelerate therapeutic efficacy, provide a structural and conceptual foundation for understanding disruptive antibody inhibition, and establish a pathway to engineering disruptive antibodies for other diseases.

Fri Dec 03 00:00:00 UTC 2021

The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab.

Nature communications

Gasser P,Tarchevskaya SS,Guntern P,Brigger D,Ruppli R,Zbären N,Kleinboelting S,Heusser C,Jardetzky TS,Eggel A

17-5899 was used in Flow cytometry/Cell sorting to investigate and compare the molecular binding profile and functional mechanisms of action of the anti-IgE antibody ligelizumab and omalizumab.

Wed Jan 08 00:00:00 UTC 2020

Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis.

Science advances

Izadi D,Layton TB,Williams L,McCann F,Cabrita M,Espirito Santo AI,Xie W,Fritzsche M,Colin-York H,Feldmann M,Midwood KS,Nanchahal J

17-5899 was used in Flow cytometry/Cell sorting to provide a detailed single-cell analysis of the immune landscape in Dupuytren's disease, a localized fibrotic condition of the hand, and identify a pathogenic signaling circuit between stromal and immune cells.

Sun Dec 01 00:00:00 UTC 2019

Basophil counts in PBMC populations during childhood acute wheeze/asthma are associated with future exacerbations.

The Journal of allergy and clinical immunology

Leffler J,Jones AC,Hollams EM,Prastanti F,Le Souëf PN,Holt PG,Bosco A,Laing IA,Strickland DH

17-5899-42 was used in Flow cytometry/Cell sorting to investigate the effect of basophils in child asthma or acute wheeze.

Thu Nov 01 00:00:00 UTC 2018

Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange.

Nature communications

Pennington LF,Tarchevskaya S,Brigger D,Sathiyamoorthy K,Graham MT,Nadeau KC,Eggel A,Jardetzky TS

17-5899 was used in Flow cytometry/Cell sorting to discover a treatment blocking basophil activation and provide a novel approach to probe mechanisms underlying IgE hypersensitivity.

Thu May 19 00:00:00 UTC 2016

Dendritic cell-bound IgE functions to restrain allergic inflammation at mucosal sites.

Mucosal immunology

Platzer B,Baker K,Vera MP,Singer K,Panduro M,Lexmond WS,Turner D,Vargas SO,Kinet JP,Maurer D,Baron RM,Blumberg RS,Fiebiger E

17-5899 was used in Flow cytometry/Cell sorting to confirm a novel immune regulatory function of Immunoglobulin E.

Fri May 01 00:00:00 UTC 2015

Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

Journal of virology

Jiang AP,Jiang JF,Wei JF,Guo MG,Qin Y,Guo QQ,Ma L,Liu BC,Wang X,Veazey RS,Ding YB,Wang JH

17-5899 was used in Flow cytometry/Cell sorting to investigate the role of mast cells in HIV-1 infection.

Wed Dec 30 00:00:00 UTC 2015

Human Blood-Circulating Basophils Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

Journal of virology

Jiang AP,Jiang JF,Guo MG,Jin YM,Li YY,Wang JH

17-5899 was used in Flow cytometry/Cell sorting to investigate the role of human granulocytes in the dissemination of HIV-1.

Sat Aug 01 00:00:00 UTC 2015

A pilot study of omalizumab in eosinophilic esophagitis.

PloS one

Loizou D,Enav B,Komlodi-Pasztor E,Hider P,Kim-Chang J,Noonan L,Taber T,Kaushal S,Limgala R,Brown M,Gupta R,Balba N,Goker-Alpan O,Khojah A,Alpan O

17-5899 was used in Flow cytometry/Cell sorting to demonstrate that anti-IgE therapy with omalizumab may result in Eosinophilic Esophagitis disease remission.

Thu Feb 04 00:00:00 UTC 2016

MHC class II expression in human basophils: induction and lack of functional significance.

PloS one

Voskamp AL,Prickett SR,Mackay F,Rolland JM,O'Hehir RE

17-5899 was used in Flow cytometry/Cell sorting to determine the antigen-presenting abilities of basophils and their role in initiating a Th2 phenotype.

Mon Sep 29 00:00:00 UTC 2014

Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood.

Current protocols in immunology

Rådinger M,Jensen BM,Kuehn HS,Kirshenbaum A,Gilfillan AM

17-5899 was used in Flow cytometry/Cell sorting to describe the relative merits and drawbacks of techniques for the development and culture of human mast cells.

Sun Aug 01 00:00:00 UTC 2010

The signal peptide of the IgE receptor alpha-chain prevents surface expression of an immunoreceptor tyrosine-based activation motif-free receptor pool.

The Journal of biological chemistry

Platzer B,Fiebiger E

17-5899 was used in Flow cytometry/Cell sorting to investigate the role of FcepsilonRI alpha-chain in immune response towards allergens.

Fri May 14 00:00:00 UTC 2010

生物信息学

蛋白别名: fc epsilon r1; Fc epsilon receptor Ia; Fc epsilon RI alpha-chain; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; Fc-epsilon RI-alpha; FcERI; high affinity immunoglobulin epsilon receptor alpha-subunit; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; RP11-550P17.3

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基因别名: FCE1A; FCER1A; FcERI

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UniProt ID: (Human) P12319

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Entrez Gene ID: (Human) 2205

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功能

IgE receptor activity IgE binding immunoglobulin receptor superfamily

参与通路