Invitrogen

Fetal Hemoglobin Monoclonal Antibody (HBF-1), FITC

产品信息

MHFH01

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

HBF-1

抗原

Human Fetal Hemoglobin (HbF)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA, <10% sucrose

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10375007

产品详细信息

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

This Fetal Hemoglobin monoclonal antibody specifically recognizes fetal hemoglobin found in fetal erythrocytes.

靶标信息

Hemorrhage of cells from an Rh+ fetus into the circulation of an Rh- mother may result in the formation of Rh-reactive antibodies in the mother. Rh hemolytic anemia in a subsequent Rh+ fetus may result from placental transfer of antibodies formed in the mother to the fetus. Although the volume of fetal erythrocytes found in the maternal circulation during pregnancy and immediately post-partum is reported to be small and of uncertain clinical significance in many cases, substantial hemorrhage may result from a number of causes including fetal or maternal trauma and placental defects. Erythrocytes containing fetal hemoglobin may be found in individuals of any age, but with lower amounts of fetal hemoglobin compared to fetal red cells. These cells have been termed F cells. High levels of F cells may also exist in adults with a heterogeneous group of genetic disorders of uncertain etiology, referred to as Hereditary Persistence of Fetal Hemoglobin. Other clinical conditions causing significant levels of anemia may also result in elevated levels of F cells. Several clinical conditions have been described with increased levels of F cells. These conditions include hereditary anemic diseases such as sickle cell anemia and thalassemia major.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

The protective effect of the spleen in sickle cell patients. A comparative study between patients with asplenia/hyposplenism and hypersplenism.

Frontiers in physiology

Peretz S,Livshits L,Pretorius E,Makhro A,Bogdanova A,Gassmann M,Koren A,Levin C

MHFH01 was used in Flow cytometry/Cell sorting to evaluate the association between the spleen status with clinical and laboratory parameters in 31 SCD patients: Group a) Patients with asplenia/hyposplenism (N = 22) (including auto-splenectomy and splenectomized patients) vs. Group b) patients with splenomegaly and or hypersplenism (N = 9).

Sat Sep 17 00:00:00 UTC 2022

ZNF410 represses fetal globin by singular control of CHD4.

Nature genetics

Vinjamur DS,Yao Q,Cole MA,McGuckin C,Ren C,Zeng J,Hossain M,Luk K,Wolfe SA,Pinello L,Bauer DE

MHFH01 was used in Flow cytometry/Cell sorting to identify zinc-finger protein (ZNF) 410 as an fetal hemoglobin repressor.

Sat May 01 00:00:00 UTC 2021

BMI1 enables extensive expansion of functional erythroblasts from human peripheral blood mononuclear cells.

Molecular therapy : the journal of the American Society of Gene Therapy

Liu S,Wu M,Lancelot M,Deng J,Gao Y,Roback JD,Chen T,Cheng L

MHFH01 was used in Flow Cytometry to report that ectopic expression of the human BMI1 gene confers extensive expansion of human erythroblasts, which can be derived readily from adult peripheral blood mononuclear cells of either healthy donors or sickle cell patients.

Wed May 05 00:00:00 UTC 2021

Hemoglobin F mitigation of sickle cell complications decreases with aging.

American journal of hematology

Tolu SS,Reyes-Gil M,Ogu UO,Thomas M,Bouhassira EE,Minniti CP

MHFH01 was used in Flow cytometry/Cell sorting to show that hemoglobin F mitigation of sickle cell complications decreases with aging.

Fri May 01 00:00:00 UTC 2020

Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease.

JCI insight

Krishnamoorthy S,Pace B,Gupta D,Sturtevant S,Li B,Makala L,Brittain J,Moore N,Vieira BF,Thullen T,Stone I,Li H,Hobbs WE,Light DR

MHFH01 was used in Flow Cytometry to investigate the ability of dimethyl fumarate to activate γ-globin transcription, and enhance foetal haemoglobin.

Thu Oct 19 00:00:00 UTC 2017

Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2.

PloS one

Shearstone JR,Golonzhka O,Chonkar A,Tamang D,van Duzer JH,Jones SS,Jarpe MB

MHFH01 was used in flow cytometry to demonstrate that chemical inhibition of HDAC1/2 induces gamma-globin and propose a role for the GATA2 gene in this process

Tue Aug 30 00:00:00 UTC 2016

Development of phenotypic screening assays for γ-globin induction using primary human bone marrow day 7 erythroid progenitor cells.

Journal of biomolecular screening

Li H,Xie W,Gore ER,Montoute MN,Bee WT,Zappacosta F,Zeng X,Wu Z,Kallal L,Ames RS,Pope AJ,Benowitz A,Erickson-Miller CL

MHFH01 was used in flow cytometry to develop a method using primary human bone marrow-derived erythroid progenitor cells to screen for compounds that induce γ-globin production.

Sun Dec 01 00:00:00 UTC 2013

Abstracts of the 16th meeting of the Clinical Cytometry Society. Clinical applications of cytometry. November 11-13, 2001. Orlando, Florida, USA.

Cytometry

No authors listed

MHFH01 was used in Flow cytometry/Cell sorting to compare CD38 (AB/C) values on CD8 T cells when a combined gate (lymphocytes and CD31 T cells) or a single gate (CD31 T cells) were applied.

Mon Oct 15 00:00:00 UTC 2001

生物信息学

蛋白别名: A-gamma globin; alpha-1 globin; gamma A hemoglobin; gamma globin; Gamma-1-globin; Hb F Agamma; hemoglobin alpha 1; Hemoglobin gamma-1 chain; Hemoglobin gamma-A chain; Hemoglobin subunit gamma-1; hemoglobin, gamma A; hemoglobin, gamma, regulator of

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基因别名: HBG-T2; HBG1; HBGA; HBGR; HSGGL1; PRO2979

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UniProt ID: (Human) P69891

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Entrez Gene ID: (Human) 3047

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功能

oxygen transporter activity iron ion binding oxygen binding heme binding

参与通路

blood coagulation oxygen transport