Product References
A specific G9a inhibitor unveils BGLT3 lncRNA as a universal mediator of chemically induced fetal globin gene expression.
Nature communications
Takase S,Hiroyama T,Shirai F,Maemoto Y,Nakata A,Arata M,Matsuoka S,Sonoda T,Niwa H,Sato S,Umehara T,Shirouzu M,Nishigaya Y,Sumiya T,Hashimoto N,Namie R,Usui M,Ohishi T,Ohba SI,Kawada M,Hayashi Y,Harada H,Yamaguchi T,Shinkai Y,Nakamura Y,Yoshida M,Ito A
MHFH05 was used in Flow cytometry/Cell sorting to find that BGLT3 long non-coding RNA plays an essential role in γ-globin induction.
Thu Jan 12 00:00:00 UTC 2023
Limitations of mouse models for sickle cell disease conferred by their human globin transgene configurations.
Disease models & mechanisms
Woodard KJ,Doerfler PA,Mayberry KD,Sharma A,Levine R,Yen J,Valentine V,Palmer LE,Valentine M,Weiss MJ
MHFH05 was used in Flow cytometry/Cell sorting to provide practical information on the genomic structures of two common mouse SCD models, illustrate their limitations for analyzing therapies to induce HbF and confirm the importance of distal DNA elements in human globin regulation.
Wed Jun 01 00:00:00 UTC 2022
Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin.
eLife
Ravi NS,Wienert B,Wyman SK,Bell HW,George A,Mahalingam G,Vu JT,Prasad K,Bandlamudi BP,Devaraju N,Rajendiran V,Syedbasha N,Pai AA,Nakamura Y,Kurita R,Narayanasamy M,Balasubramanian P,Thangavel S,Marepally S,Velayudhan SR,Srivastava A,DeWitt MA,Crossley M,Corn JE,Mohankumar KM
MHFH05 was used in Flow cytometry/Cell sorting to shed light on so far unknown regulatory elements within the HBG promoter and identified additional targets for therapeutic upregulation of fetal hemoglobin.
Fri Feb 11 00:00:00 UTC 2022
Genome Engineering of Hematopoietic Stem Cells Using CRISPR/Cas9 System.
Methods in molecular biology (Clifton, N.J.)
Devaraju N,Rajendiran V,Ravi NS,Mohankumar KM
MHFH05 was used in Flow cytometry/Cell sorting to describe the detailed methodology for the CRISPR-Cas9 mediated gene knockout, deletion, addition, and correction in human HSPCs by viral and nonviral approaches and present a comprehensive protocol for the analysis of genome modified HSPCs toward the erythroid and megakaryocyte lineage in vitro and the long-term multilineage reconstitution capacity in the recently developed NBSGW mouse model that supports human erythropoiesis.
Mon May 09 00:00:00 UTC 2022
Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells.
Nature genetics
Qin K,Huang P,Feng R,Keller CA,Peslak SA,Khandros E,Saari MS,Lan X,Mayuranathan T,Doerfler PA,Abdulmalik O,Giardine B,Chou ST,Shi J,Hardison RC,Weiss MJ,Blobel GA
MHFH05 was used in flow cytometry to identify via a CRISPR-Cas9 genetic screen two members of the NFI transcription factor family-NFIA and NFIX-as HBG1/2 repressors.
Wed Jun 01 00:00:00 UTC 2022
Understanding heterogeneity of fetal hemoglobin induction through comparative analysis of F and A erythroblasts.
Blood
Khandros E,Huang P,Peslak SA,Sharma M,Abdulmalik O,Giardine BM,Zhang Z,Keller CA,Hardison RC,Blobel GA
MHFH05 was used in Flow Cytometry to understand the heterogeneity of HbF activation by developing techniques to purify and profile differentiation stage-matched late erythroblast F cells and non-F cells (A cells) from the human HUDEP2 erythroid cell line and primary human erythroid cultures.
Thu May 28 00:00:00 UTC 2020
HRI depletion cooperates with pharmacologic inducers to elevate fetal hemoglobin and reduce sickle cell formation.
Blood advances
Peslak SA,Khandros E,Huang P,Lan X,Geronimo CL,Grevet JD,Abdulmalik O,Zhang Z,Giardine BM,Keller CA,Shi J,Hardison RC,Blobel GA
MHFH05 was used in Flow cytometry/Cell sorting to provide a foundation for the exploration of combining future small-molecule HRI inhibitors with additional pharmacologic HbF inducers to maximize HbF production and preserve erythroid cell functionality for the treatment of SCD and other hemoglobinopathies.
Tue Sep 22 00:00:00 UTC 2020
ATF4 Regulates MYB to Increase γ-Globin in Response to Loss of β-Globin.
Cell reports
Boontanrart MY,Schröder MS,Stehli GM,Banović M,Wyman SK,Lew RJ,Bordi M,Gowen BG,DeWitt MA,Corn JE
MHFH05 was used in Flow cytometry/Cell sorting to model the stress caused by reduced levels of adult β-globin using CRISPR-Cas genome editing.
Tue Aug 04 00:00:00 UTC 2020
Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci.
Nature genetics
Canver MC,Lessard S,Pinello L,Wu Y,Ilboudo Y,Stern EN,Needleman AJ,Galactéros F,Brugnara C,Kutlar A,McKenzie C,Reid M,Chen DD,Das PP,A Cole M,Zeng J,Kurita R,Nakamura Y,Yuan GC,Lettre G,Bauer DE,Orkin SH
MHFH05 was used in Flow cytometry/Cell sorting to demonstrate the use of multiple designer nucleases and variant-aware library design to interrogate trait-associated regulatory DNA at high resolution.
Sat Apr 01 00:00:00 UTC 2017
Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
The Journal of clinical investigation
Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP
MHFH05 was used in Flow cytometry/Cell sorting to demonstrate that endothelial Notch ligands promote PSC-definitive haematopoiesis and production of long-term engrafting CD34+ cells.
Mon Mar 02 00:00:00 UTC 2015
Reactivation of developmentally silenced globin genes by forced chromatin looping.
Cell
Deng W,Rupon JW,Krivega I,Breda L,Motta I,Jahn KS,Reik A,Gregory PD,Rivella S,Dean A,Blobel GA
MHFH05 was used in immunocytochemistry to show that forced chromatin looping can override a stringent developmental gene expression program
Thu Aug 14 00:00:00 UTC 2014
Proposed flow cytometric reference method for the determination of erythroid F-cell counts.
Cytometry
Chen JC,Bigelow N,Davis BH
MHFH05 was used in flow cytometry and immunocytochemistry to assess the feasibility of using the orange autofluorescence signal to detect fetal hemoglobin
Tue Aug 15 00:00:00 UTC 2000
Simplified flow cytometric method for fetal hemoglobin containing red blood cells.
Cytometry
Mundee Y,Bigelow NC,Davis BH,Porter JB
MHFH05 was used in flow cytometry to describe a new method to detect fetal hemoglobin
Fri Dec 15 00:00:00 UTC 2000
Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis.
British journal of haematology
Thorpe SJ,Thein SL,Sampietro M,Craig JE,Mahon B,Huehns ER
MHFH05 was used in flow cytometry to develop immunostaining methods to measure intracellular hemoglobin in erythrocytes by fluorescence activated cell flow cytometry
Sun May 01 00:00:00 UTC 1994
Quantitation of fetal-maternal hemorrhage by flow cytometry. A simple and accurate method.
American journal of clinical pathology
Nance SJ,Nelson JM,Arndt PA,Lam HC,Garratty G
MHFH05 was used in flow cytometry to develop a flow cytometry-based method to measure fetal-maternal hemorrhage
Wed Mar 01 00:00:00 UTC 1989
