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Ffar4 Antibody (600-401-GW0) in WB
Western Blot of Rabbit Anti-Ffar4 antibody. Lane 1: Ladder (Opal Prestained) p/n MB-210-0500. Lane 2: Raw 264.7 WCL (p/n W10-001-369). Lane 3: NIH/3T3 WCL (p/n W10-000-358). Lane 4: PC-12 WCL (p/n W12-001-GL9). Lane 5: Ladder (Opal Prestained) p/n MB-210-0500. Load: 35 µg per lane. Primary antibody: Ffar4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP secondary antibody 611-103-122 at 1:70,000 for 30 min at RT. Block: MB-073 BlockOut Universal Blocking Buffer for 30 min at RT. Predicted/Observed size: 40.8 kDa, ... View More
产品信息
600-401-GW0
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Affinity purified Anti-Ffar4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-terminus portion of mouse Ffar4 protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Ffar4 is directed against mouse Ffar4 at a n-terminal position. A BLAST analysis was used to suggest reactivity with this protein in rat based on 100% homology match for the immunogen sequence.
靶标信息
An EST for GPR120 has been isolated from a human colon cancer library. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Free fatty acid receptor 4; G protein-coupled receptor 120; G-protein coupled receptor 120; G-protein coupled receptor GT01; G-protein-coupled receptor GT01; GPCR120; Omega-3 fatty acid receptor 1
基因别名: AI552415; Ffar4; Gpr120; Gpr129; GT01; KPG_013; O3far1; Pgr4
UniProt ID: (Mouse) Q7TMA4
Entrez Gene ID: (Mouse) 107221
signal transduction
G-protein coupled receptor signaling pathway
regulation of glucose transport
negative regulation of apoptotic process
hormone secretion
negative regulation of cytokine secretion
negative regulation of inflammatory response
detection of chemical stimulus involved in sensory perception of taste
positive regulation of ERK1 and ERK2 cascade
