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Fibroblast Activation Protein Antibody (600-401-A73) in WB
Western blot using Rocklands affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular... View More
产品信息
600-401-A73
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.
偶联物
形式
Liquid
浓度
1.1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with FAP from other sources has not been determined.
靶标信息
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Dipeptidyl peptidase FAP; FAPalpha; Fibroblast activation protein alpha; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Prolyl endopeptidase FAP; Seprase; Serine integral membrane protease; SIMP; Surface-expressed protease
基因别名: Fap; SIMP
UniProt ID: (Mouse) P97321
Entrez Gene ID: (Mouse) 14089
angiogenesis
proteolysis
apoptotic process
cell adhesion
regulation of collagen catabolic process
negative regulation of extracellular matrix disassembly
endothelial cell migration
proteolysis involved in cellular protein catabolic process
negative regulation of cell proliferation involved in contact inhibition
positive regulation of cell cycle arrest
mitotic cell cycle arrest
melanocyte proliferation
positive regulation of execution phase of apoptosis
melanocyte apoptotic process
negative regulation of extracellular matrix organization
