Invitrogen

Filamin A alpha-2 Polyclonal Antibody

1 Published Figure
1 Reference
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-29598

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
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Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1769 and 2098 of Human Filamin A alpha 2
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547074

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse kidney, PC-12, Rat2.

Predicted reactivity: Mouse (98%), Rat (98%), Rabbit (98%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ABP-280; actin binding protein 280; Actin-binding protein 280; alpha filamin; Alpha-filamin; dilated pupils 2; Endothelial actin-binding protein; Endothelial actin-binding protein 280); filamin A, alpha; filamin, alpha; Filamin-1; Filamin-A; FLN-A; Non-muscle filamin

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Gene Aliases: ABP-280; ABPX; CSBS; CVD1; Dilp2; F730004A14Rik; filamin-1; FLN; FLN-A; FLN1; FLNA; FMD; GENA 379; MNS; NHBP; OPD; OPD1; OPD2; RGD1560614; XLVD; XMVD

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UniProt ID: (Human) P21333

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Entrez Gene ID: (Human) 2316, (Rat) 293860, (Mouse) 192176

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Function(s)
G-protein coupled receptor binding glycoprotein binding signal transducer activity protein binding transcription factor binding Rho GTPase binding Ral GTPase binding kinase binding small GTPase binding mu-type opioid receptor binding Fc-gamma receptor I complex binding protein homodimerization activity poly(A) RNA binding SMAD binding Rac GTPase binding actin filament binding GTPase binding cadherin binding involved in cell-cell adhesion protein kinase C binding protein complex binding actin binding
Process(es)
epithelial to mesenchymal transition adenylate cyclase-inhibiting dopamine receptor signaling pathway biological_process actin cytoskeleton organization actin cytoskeleton reorganization protein localization to cell surface negative regulation of protein catabolic process cilium assembly mRNA transcription from RNA polymerase II promoter positive regulation of transcription factor import into nucleus receptor clustering positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of sequence-specific DNA binding transcription factor activity early endosome to late endosome transport establishment of protein localization protein stabilization cytoplasmic sequestering of protein actin crosslink formation platelet aggregation mitotic spindle assembly negative regulation of transcription from RNA polymerase I promoter cell projection organization negative regulation of apoptotic process positive regulation of substrate adhesion-dependent cell spreading positive regulation of integrin-mediated signaling pathway platelet degranulation platelet activation regulation of cell migration cell junction assembly wound healing, spreading of cells semaphorin-plexin signaling pathway
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