Bethyl Laboratories

Flotillin-1 Polyclonal Antibody

查看其他19个Flotillin 1抗体

抗体检测数据 (1)

Flotillin-1 Antibody in Western Blot (WB)

Flotillin-1 Antibody in Western Blot (WB)

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Flotillin-1 Antibody (A303-422A-T) in WB

Detection of human and mouse Flotillin-1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody (Product # A303-422A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody A303-421A, which recognizes an upstream epitope. Detection: Ch... View More

Flotillin-1 Antibody in Western Blot (WB)

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Flotillin-1 Antibody in Western Blot (WB) — Detection of human and mouse Flotillin-1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody (Product # A303-422A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody A303-421A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

产品信息

A303-422A-T

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

-

免疫沉淀 (IP)

2-10 µg/mg lysate

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 377 and 427 of human Flotillin 1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

tris citrate/phosphate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Bovine, Pig, Orangutan, Monkey and Chimpanzee

靶标信息

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DADB-118P11.2; Flotillin-1; integral membrane component of caveolae; OTTHUMP00000164855

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基因别名: FLOT1; reggie-2

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UniProt ID: (Human) O75955, (Mouse) O08917

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Entrez Gene ID: (Human) 10211, (Mouse) 14251

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protease binding protein binding ionotropic glutamate receptor binding protein heterodimerization activity

membrane raft assembly positive regulation of cytokine production positive regulation of protein phosphorylation regulation of receptor internalization endocytosis axonogenesis axon guidance extracellular matrix disassembly positive regulation of protein binding positive regulation of interferon-beta production dsRNA transport positive regulation of heterotypic cell-cell adhesion positive regulation of toll-like receptor 3 signaling pathway response to endoplasmic reticulum stress regulation of Rho protein signal transduction plasma membrane raft assembly positive regulation of endocytosis positive regulation of skeletal muscle tissue development protein homooligomerization positive regulation of cell adhesion molecule production protein kinase C signaling cellular response to exogenous dsRNA protein localization to plasma membrane establishment of protein localization to plasma membrane positive regulation of myoblast fusion positive regulation of cell junction assembly protein localization to membrane raft positive regulation of cell-cell adhesion mediated by cadherin positive regulation of synaptic transmission, dopaminergic regulation of toll-like receptor 4 signaling pathway