Invitrogen

Flotillin 1 Polyclonal Antibody

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 8

Flotillin 1 Antibody (PA5-18053) in IHC (P)

Immunohistochemistry (PFA fixed) analysis of Flotillin 1 using Flotillin 1 Polyclonal Antibody (Product # PA5-18053) (5 µg/mL) in staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Flotillin 1 Antibody in Western Blot (WB)

Product Image Gallery

产品信息

PA5-18053

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.3 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (SISQVNHKPLRTA) corresponding to the C-terminus amino acids of FLOT1 (aa 415-427).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_10979951

产品详细信息

This antibody is predicted to react with bovine, canine and porcine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

靶标信息

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Muscle cells of sporadic amyotrophic lateral sclerosis patients secrete neurotoxic vesicles.

Journal of cachexia, sarcopenia and muscle

Le Gall L,Duddy WJ,Martinat C,Mariot V,Connolly O,Milla V,Anakor E,Ouandaogo ZG,Millecamps S,Lainé J,Vijayakumar UG,Knoblach S,Raoul C,Lucas O,Loeffler JP,Bede P,Behin A,Blasco H,Bruneteau G,Del Mar Amador M,Devos D,Henriques A,Hesters A,Lacomblez L,Laforet P,Langlet T,Leblanc P,Le Forestier N,Maisonobe T,Meininger V,Robelin L,Salachas F,Stojkovic T,Querin G,Dumonceaux J,Butler Browne G,González De Aguilar JL,Duguez S,Pradat PF

PA5-18053 was used in Western Blot to test a hypothesis that muscle vesicles may be involved in ALS pathology.

Fri Apr 01 00:00:00 UTC 2022

The Metalloprotease-Disintegrin ADAM8 Alters the Tumor Suppressor miR-181a-5p Expression Profile in Glioblastoma Thereby Contributing to Its Aggressiveness.

Frontiers in oncology

Schäfer A,Evers L,Meier L,Schlomann U,Bopp MHA,Dreizner GL,Lassmann O,Ben Bacha A,Benescu AC,Pojskic M,Preußer C,von Strandmann EP,Carl B,Nimsky C,Bartsch JW

Published figure using Flotillin 1 polyclonal antibody (Product # PA5-18053) in Western Blot

Tue Apr 05 00:00:00 UTC 2022

ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9.

International journal of molecular sciences

Cook L,Sengelmann M,Winkler B,Nagl C,Koch S,Schlomann U,Slater EP,Miller MA,von Strandmann EP,Dörsam B,Preußer C,Bartsch JW

Published figure using Flotillin 1 polyclonal antibody (Product # PA5-18053) in Western Blot

Thu Feb 10 00:00:00 UTC 2022

Extracellular Vesicle-Based Detection of Pancreatic Cancer.

Frontiers in cell and developmental biology

Verel-Yilmaz Y,Fernández JP,Schäfer A,Nevermann S,Cook L,Gercke N,Helmprobst F,Jaworek C,Pogge von Strandmann E,Pagenstecher A,Bartsch DK,Bartsch JW,Slater EP

PA5-18053 was used in Western Blot to conclude that detection of ADAM8-positive EVs and related cargo miR-720 and miR-451 may constitute a specific biomarker set for screening individuals at risk for PDAC.

Tue Aug 10 00:00:00 UTC 2021

Optimized method for extraction of exosomes from human primary muscle cells.

Skeletal muscle

Le Gall L,Ouandaogo ZG,Anakor E,Connolly O,Butler Browne G,Laine J,Duddy W,Duguez S

PA5-18053 was used in Western Blotting to optimise conditions for the in vitro cultivation of human myoblasts, and the quality and yield of exosomes extracted using an ultracentrifugation protocol are compared with a modified polymer-based precipitation strategy combined with extra washing steps.