Invitrogen

Flotillin 1 Polyclonal Antibody

Flotillin 1 Antibody in Western Blot (WB)

图: 1 / 4

Flotillin 1 Antibody (PA5-19713) in WB

Western blot was performed using Anti- Flotillin 1 Polyclonal Antibody (Product # PA5-19713) and a 47kDa band corresponding to Flotillin 1 was observed across all the cell lines and tissue lysates tested except THP1 which is reported negative for Flotillin 1 expression. An uncharacterized band was observed at ~32KDa across all the samples and ~60KDa in THP-1 sample alone. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), A549 (Lane 6), THP-1 (Lane 7), tissue extracts... View More

Product Image Gallery

产品信息

PA5-19713

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Flotillin 1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_10981198

产品详细信息

For Western Blot, this antibody has non-specific bands at 35 kDa.

Heat mediated antigen retrieval recommended prior to tissue staining.

This antibody is predicted to react with many species based on sequence homology.

Store antibody at 4ºC for 1-2 weeks. For long-term storage, store at -20ºC.

靶标信息

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.

Nature cell biology

Kugeratski FG,Hodge K,Lilla S,McAndrews KM,Zhou X,Hwang RF,Zanivan S,Kalluri R

PA5-19713 was used in Simple Western - Size to provide a comprehensive quantitative atlas of core proteins ubiquitous to exosomes that can serve as a resource for the scientific community.

Tue Jun 01 00:00:00 UTC 2021

The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate blood.

Biology open

Abhinav K,Feng L,Morrison E,Jung Y,Dear J,Takahashi S,Heck MMS

PA5-19713 was used in Western Blotting to show that the protease motif of invadolysin is important for its role in lipid accumulation, but not in glycogen accumulation.