Invitrogen

Flotillin 1 Recombinant Rabbit Monoclonal Antibody (ARC0765)

查看其他19个Flotillin 1抗体

抗体检测数据 (5)

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

Flotillin 1 Antibody (MA5-35364) in IHC (P)

Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded human breast cancer using. Samples were incubated with Flotillin 1 Monoclonal antibody (Product # MA5-35364) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Flotillin 1 Antibody in Western Blot (WB)

Flotillin 1 Antibody in Western Blot (WB)

Product Image Gallery

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded human breast cancer using. Samples were incubated with Flotillin 1 Monoclonal antibody (Product # MA5-35364) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse heart. Samples were incubated with Flotillin 1 Monoclonal antibody (Product # MA5-35364) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Flotillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart. Samples were incubated with Flotillin 1 Monoclonal antibody (Product # MA5-35364) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Flotillin 1 Antibody in Western Blot (WB) — Western blot analysis of Flotillin 1 in various lysates. Samples were incubated with Flotillin 1 Monoclonal antibody (Product # MA5-35364) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.

Flotillin 1 Antibody in Western Blot (WB) — Western blot analysis of Flotillin 1 in various lysates. Samples were incubated with Flotillin 1 Monoclonal antibody (Product # MA5-35364) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

产品信息

MA5-35364

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:800

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC0765

抗原

A synthetic peptide corresponding to a sequence within amino acids 328-427 of human Flotillin 1 (O75955)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.6 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2849265

产品详细信息

Immunogen sequence: GARARAEAEQ MAKKAEAFQL YQEAAQLDML LEKLPQVAEE ISGPLTSANK ITLVSSGSGT MGAAKVTGEV LDILTRLPES VERLTGVSIS QVNHKPLRTA

靶标信息

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DADB-118P11.2; Flotillin-1; integral membrane component of caveolae; OTTHUMP00000164855; REG-2; Reggie-2

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基因别名: FLOT1; Rareg; Reg2; reggie-2

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UniProt ID: (Human) O75955, (Mouse) O08917, (Rat) Q9Z1E1

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Entrez Gene ID: (Human) 10211, (Mouse) 14251, (Rat) 64665

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protease binding protein binding ionotropic glutamate receptor binding protein heterodimerization activity

membrane raft assembly positive regulation of cytokine production positive regulation of protein phosphorylation regulation of receptor internalization endocytosis axonogenesis axon guidance extracellular matrix disassembly positive regulation of protein binding positive regulation of interferon-beta production dsRNA transport positive regulation of heterotypic cell-cell adhesion positive regulation of toll-like receptor 3 signaling pathway response to endoplasmic reticulum stress regulation of Rho protein signal transduction plasma membrane raft assembly positive regulation of endocytosis positive regulation of skeletal muscle tissue development protein homooligomerization positive regulation of cell adhesion molecule production protein kinase C signaling cellular response to exogenous dsRNA protein localization to plasma membrane establishment of protein localization to plasma membrane positive regulation of myoblast fusion positive regulation of cell junction assembly protein localization to membrane raft positive regulation of cell-cell adhesion mediated by cadherin positive regulation of synaptic transmission, dopaminergic regulation of toll-like receptor 4 signaling pathway