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Fyn Antibody (MA1-19331) in ICC/IF
Immunocytochemistry analysis of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green) Monoclonal antibody (Product # MA1-19331) using a dilution of 5 µg/mL. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
产品信息
MA1-19331
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Hamster,
Human,
Mouse
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
FYN-01
抗原
Recombinant fragment of human Fyn (aa 7-176)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1074491
产品详细信息
This antibody recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).
Immunoprecipitation: Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitants Fyn from cell lysates.
靶标信息
FYN is a SRC-related kinase expressed in brain, skeletal muscle and adipose tissues. Fyn functions in a large number of pathways and has been shown to play a role in memory. FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C syn protooncogene; c-syn protooncogene; EC 2.7.10.2; FYN oncogene related to SRC, FGR, YES; kinase Fyn; MGC45350; OKT3-induced calcium influx regulator; OTTHUMP00000017916; OTTHUMP00000017917; P59 p59-Fyn; p59-Fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; proto-oncogene tyrosine-protein kinase Fyn; Protooncogene Syn; RP1-66H14.1; SLK; Src Kinase p59; Src like kinase; Src-like kinase; src/yes-related novel; tyrosine kinase p59fyn(T); Tyrosine-protein kinase Fyn
基因别名: AI448320; AW552119; FYN; p59-FYN; SLK; SYN
UniProt ID: (Human) E1P557, (Mouse) P39688
Entrez Gene ID: (Human) 2534, (Mouse) 14360
G-protein coupled receptor binding
glycoprotein binding
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
protein binding
ATP binding
tubulin binding
T cell receptor binding
CD4 receptor binding
CD8 receptor binding
phosphatidylinositol 3-kinase binding
ion channel binding
metal ion binding
ephrin receptor binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
peptide hormone receptor binding
growth factor receptor binding
nucleotide binding
protein kinase activity
receptor binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein complex binding
MAPK cascade
neuron migration
stimulatory C-type lectin receptor signaling pathway
adaptive immune response
protein phosphorylation
calcium ion transport
transmembrane receptor protein tyrosine kinase signaling pathway
axon guidance
central nervous system development
blood coagulation
learning
feeding behavior
regulation of cell shape
negative regulation of gene expression
positive regulation of neuron projection development
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
viral process
cell migration
peptidyl-tyrosine phosphorylation
cell differentiation
platelet activation
forebrain development
T cell costimulation
negative regulation of protein ubiquitination
intracellular signal transduction
cellular response to platelet-derived growth factor stimulus
peptidyl-tyrosine autophosphorylation
Fc-gamma receptor signaling pathway involved in phagocytosis
T cell activation
regulation of cell proliferation
negative regulation of protein catabolic process
response to drug
positive regulation of tyrosine phosphorylation of Stat5 protein
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
innate immune response
response to ethanol
phosphatidylinositol phosphorylation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
phosphatidylinositol-mediated signaling
dendrite morphogenesis
regulation of defense response to virus by virus
activated T cell proliferation
T cell receptor signaling pathway
leukocyte migration
detection of mechanical stimulus involved in sensory perception of pain
cellular response to peptide hormone stimulus
cellular response to transforming growth factor beta stimulus
positive regulation of protein localization to nucleus
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
immune system process
cell surface receptor signaling pathway
multicellular organism development
phosphorylation
myelination
protein autophosphorylation
