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G3BP1 Antibody (MA5-50804) in ICC/IF
Immunocytochemistry analysis of G3BP1 in LOVO cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with G3BP1 recombinant monoclonal antibody (Product # MA5-50804) with a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the pri... View More
产品信息
MA5-50804
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE35-20
抗原
Recombinant protein within Human G3BP aa 367-466/466.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3093724
产品详细信息
Positive control: A549 cell lysate, N2A cell lysate, human colon tissue, mouse brain tissue, rat cerebellum tissue, A549, LOVO, N2A.
Predicted band size: 52 kDa
Subcellular Location: Cytoplasm, Nucleus.
靶标信息
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP
基因别名: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666
UniProt ID: (Human) Q13283, (Mouse) P97855
Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041
DNA binding
mRNA binding
ATP-dependent DNA helicase activity
ATP-dependent RNA helicase activity
endonuclease activity
protein binding
ATP binding
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
helicase activity
nuclease activity
hydrolase activity
RNA metabolism protein
nucleic acid metabolism protein
