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GAD65 Antibody (PA5-77983) in IHC (P)
GAD65 Polyclonal Antibody detects GAD65 protein expression by immunohistochemical analysis. Sample: Paraffin-Embedded adult mouse retina. Green: GAD65 protein stained by GAD65 Polyclonal Antibody (Product # PA5-77983) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 mi... View More
产品信息
PA5-77983
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the N-terminus region of mouse GAD65. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.34 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735818
产品详细信息
Positive Control: mouse brain, rat brain
Predicted Reactivity: Dog (81%), Rhesus Monkey (80%), Bovine (81%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
GAD65 (glutamic acid decarboxylase-65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; MGC161605; MGC161607; RP11-420F12.2
基因别名: 6330404F12Rik; GAD(65); Gad-2; Gad2; GAD65
UniProt ID: (Mouse) P48320, (Rat) Q05683
Entrez Gene ID: (Mouse) 14417, (Rat) 24380
