Invitrogen

GADD34 Polyclonal Antibody

GADD34 Antibody in Immunocytochemistry (ICC/IF)

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GADD34 Antibody (PA1-139) in ICC/IF

Immunofluorescent analysis of GADD34 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a GADD34 polyclonal antibody (Product # PA1-139) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:500 for 30 minutes at room t... View More

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产品信息

PA1-139

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看6篇已发表文章 6篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:100

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to amino acids C R(595) I T Q A Q E E L S P C L T P A A R(612) from human GADD34

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2539894

产品详细信息

Western blot analysis of PA1-139 detects an ~73 kDa protein in HeLa cells. PA1-139 shows specificity to GADD34 and can be blocked by incubating with an abundance of the immunizing peptide.

靶标信息

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

4-phenylbutyric acid-Identity crisis; can it act as a translation inhibitor?

Aging cell

Stein D,Slobodnik Z,Tam B,Einav M,Akabayov B,Berstein S,Toiber D

PA1-139 was used in Western Blot to show that 4PBA is a conserved weak inhibitor of mRNA translation, both in vitro and in cellular systems, and furthermore-it does not promote protein folding nor prevents aggregation.

Thu Dec 01 00:00:00 UTC 2022

Inhibition of eIF2α Phosphorylation by Peste des Petits Ruminant Virus Phosphoprotein Facilitates Viral Replication.

Frontiers in veterinary science

Alfred N,Qian B,Qin X,Yin X,Prajapati M,Dou Y,Li Y,Zhang Z

PA1-139 was used in Immunocytochemistry to investigate molecular mechanisms associated with peste des petits ruminant virus replication and host cell interaction.

Sat Jul 24 00:00:00 UTC 2021

Inhibition of eIF2α Phosphorylation by Peste des Petits Ruminant Virus Phosphoprotein Facilitates Viral Replication.

Frontiers in veterinary science

Alfred N,Qian B,Qin X,Yin X,Prajapati M,Dou Y,Li Y,Zhang Z

PA1-139 was used in Immunocytochemistry to investigate molecular mechanisms associated with peste des petits ruminant virus replication and host cell interaction.

Sat Jul 24 00:00:00 UTC 2021

Inhibition of eIF2α Phosphorylation by Peste des Petits Ruminant Virus Phosphoprotein Facilitates Viral Replication.

Frontiers in veterinary science

Alfred N,Qian B,Qin X,Yin X,Prajapati M,Dou Y,Li Y,Zhang Z

PA1-139 was used in Immunocytochemistry to investigate molecular mechanisms associated with peste des petits ruminant virus replication and host cell interaction.

Sat Jul 24 00:00:00 UTC 2021

Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cells.

Cell death & disease

Attwood KM,Robichaud A,Westhaver LP,Castle EL,Brandman DM,Balgi AD,Roberge M,Colp P,Croul S,Kim I,McCormick C,Corcoran JA,Weeks A

PA1-139 was used in Western Blot to indicate that modulating the stress response can be used to exploit the hypoxic niche of GBM tumors, causing cell death by disrupting pro-survival stress responses and control of protein synthesis.

Tue Nov 17 00:00:00 UTC 2020

Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cells.

Cell death & disease

Attwood KM,Robichaud A,Westhaver LP,Castle EL,Brandman DM,Balgi AD,Roberge M,Colp P,Croul S,Kim I,McCormick C,Corcoran JA,Weeks A

Tue Nov 17 00:00:00 UTC 2020

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

PA1-139 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 UTC 2020

GADD34 is a modulator of autophagy during starvation.

Science advances

Gambardella G,Staiano L,Moretti MN,De Cegli R,Fagnocchi L,Di Tullio G,Polletti S,Braccia C,Armirotti A,Zippo A,Ballabio A,De Matteis MA,di Bernardo D

PA1-139 was used in Immunocytochemistry-immunoflourescence to show TFEB directly activates expression of GADD34 to suppress protein synthesis inhibition.

Tue Sep 01 00:00:00 UTC 2020

EIF1AX and RAS Mutations Cooperate to Drive Thyroid Tumorigenesis through ATF4 and c-MYC.

Cancer discovery

Krishnamoorthy GP,Davidson NR,Leach SD,Zhao Z,Lowe SW,Lee G,Landa I,Nagarajah J,Saqcena M,Singh K,Wendel HG,Dogan S,Tamarapu PP,Blenis J,Ghossein RA,Knauf JA,Rätsch G,Fagin JA

PA1-139 was used in Western Blotting to describe the identification of the key signaling drivers of transformation by EIF1AX mutants, particularly EIF1AX-A113splice, alone and in the context of RAS, and the therapeutic dependencies they confer.

Fri Feb 01 00:00:00 UTC 2019

Heterozygous Truncating Variants in POMP Escape Nonsense-Mediated Decay and Cause a Unique Immune Dysregulatory Syndrome.

American journal of human genetics

Poli MC,Ebstein F,Nicholas SK,de Guzman MM,Forbes LR,Chinn IK,Mace EM,Vogel TP,Carisey AF,Benavides F,Coban-Akdemir ZH,Gibbs RA,Jhangiani SN,Muzny DM,Carvalho CMB,Schady DA,Jain M,Rosenfeld JA,Emrick L,Lewis RA,Lee B,Zieba BA,Küry S,Krüger E,Lupski JR,Bostwick BL,Orange JS

PA1-139 was used in Western Blotting to characterise the Proteasome maturation protein-related autoinflammation and immune dysregulation disease discovered in two unrelated individuals with a unique constellation of early-onset combined immunodeficiency, inflammatory neutrophilic dermatosis, and autoimmunity.

Thu Jun 07 00:00:00 UTC 2018

生物信息学

蛋白别名: Growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; myeloid differentiation primary response gene 116; Myeloid differentiation primary response protein MyD116; Myeloid differentiation primary response protein MyD116 homolog; PR15A; Protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A; protein phosphatase 1, regulatory subunit 15A

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基因别名: 9630030H21; GADD34; Myd116; PPP1R15A

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UniProt ID: (Human) O75807, (Mouse) P17564

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Entrez Gene ID: (Human) 23645, (Mouse) 17872

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功能

protein binding protein phosphatase 1 binding protein phosphatase type 1 regulator activity protein kinase binding protein phosphatase type 1 activator activity protein phosphatase regulator activity protein phosphatase binding

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