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GALK1 Antibody (PA5-80652) in ICC/IF
Immunofluorescence staining of HG3C-GALK1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with GALK1 Polyclonal Antibody (Product # PA5-80652, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Product Details
PA5-80652
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human GALK1 Protein (Met1-Leu392)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787944
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human GALK1.
Target Information
Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts in the adult population. GALK1 sequence shares the greatest level of conservation, 44. 5% identity with that from E. coli and 34. 6% amino acid identity with the product of the human GALK2 gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 19; Galactokinase; Galactose kinase
Gene Aliases: GALK; GALK1; GK1; HEL-S-19
UniProt ID: (Human) P51570
Entrez Gene ID: (Human) 2584
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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