Invitrogen

GAP43 Recombinant Rabbit Monoclonal Antibody (SC60-06)

GAP43 Antibody in Immunocytochemistry (ICC/IF)

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GAP43 Antibody (MA5-32256) in ICC/IF

Immunocytochemical analysis of GAP43 in SHG-44 cells using a GAP43 Monoclonal antibody (Product # MA5-32256) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

GAP43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GAP43 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested for GAP43 expression owing to their inherent genetic constitution. Relative expression of GAP43 was observed in SH-SY5Y differentiated neurons and other brain-specific cell lines and tissues but not in A549, A-431 and Mouse lung which are reported to be low to negative in GAP43 expression using Anti-GAP43 Recombinant Rabbit Monoclonal Antibody (SC60-06) (Product # MA5-32256) in Western Blot. {RE}

产品信息

MA5-32256

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:100,000

免疫组化（石蜡） (IHC (P))

1:10000-1:50000

免疫细胞化学 (ICC/IF)

1:500

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SC60-06

抗原

Synthetic peptide within Human GAP43 aa 189-238

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809542

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

GAP43 (Neuromodulin) is associated with nerve growth. GAP43 is expressed by developing and regenerating neurons, and to a lesser extent reactive glial cells. It is a major component of the motile "growth cones" that form the tips of elongating axons. GAP43 also plays a role in axonal and dendritic filopodia induction.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Axonal membrane protein GAP-43; brain abundant, membrane attached signal protein 2; Calmodulin-binding protein P-57; growth accentuating protein 43; Growth-associated protein 43; MGC156666; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; neuron protein F1; pp46; Protein F1

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基因别名: B-50; Basp2; GAP-43; GAP43; PP46

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UniProt ID: (Human) P17677, (Rat) P07936, (Mouse) P06837

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Entrez Gene ID: (Human) 2596, (Rat) 29423, (Mouse) 14432

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功能

phosphatidylserine binding protein binding calmodulin binding lysophosphatidic acid binding phosphatidylinositol phosphate binding

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