Invitrogen

GAPDH Monoclonal Antibody (3E8AD9)

GAPDH Antibody in Immunocytochemistry (ICC/IF)

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GAPDH Antibody (A21994) in ICC/IF

Immunofluorescence analysis of GAPDH was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GAPDH Polyclonal Antibody (Product # A21994) at 5µg/mL in 0.1% BSA and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature... View More

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GAPDH Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with alpha GAPDH siRNA and loss of signal was observed in Western Blot using Anti-GAPDH Monoclonal Antibody (Product # A21994). {KD}

产品信息

A21994

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.125 µg/mL

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免疫细胞化学 (ICC/IF)

5 µg/mL

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

3E8AD9

抗原

Purified GAPDH from human erythrocytes, purified PC from bovine liver.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Wet ice

AB_2535905

靶标信息

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Orai1 downregulation causes proliferation reduction and cell cycle arrest via inactivation of the Ras-NF-κB signaling pathway in osteoblasts.

BMC musculoskeletal disorders

Guo Y,Fan J,Liu S,Hao D

A21994 was used in Western Blot to reveal that Orai1 downregulation may reduce cyclin D1 expression by inactivating the Ras-NF-κB signaling pathway thus blocking osteoblast proliferation and cell cycle.

Mon Apr 11 00:00:00 UTC 2022

A versatile EPR toolbox for the simultaneous measurement of oxygen consumption and superoxide production.

Redox biology

Danhier P,Northshield H,Isenborghs P,Jordan BF,Gallez B

A21994 was used in Western Blotting to describe an assay to analyze simultaneously the oxygen consumption rate (OCR) and superoxide production in a biological system.

Thu Apr 01 00:00:00 UTC 2021

A versatile EPR toolbox for the simultaneous measurement of oxygen consumption and superoxide production.

Redox biology

Danhier P,Northshield H,Isenborghs P,Jordan BF,Gallez B

A21994 was used in Western Blotting to describe an assay to analyze simultaneously the oxygen consumption rate (OCR) and superoxide production in a biological system.

Thu Apr 01 00:00:00 UTC 2021

Synergetic Effect of 4-Phenylbutyric Acid in Combination with Cyclosporine A on Cardiovascular Function in Sepsis Rats via Inhibition of Endoplasmic Reticulum Stress and Mitochondrial Permeability Transition Pore Opening.

Frontiers in pharmacology

Kuang L,Zhu Y,Wu Y,Peng X,Tian K,Liu L,Li T

A21994 was used in Immunocytochemistry, Immunoprecipitation, Western Blot to show that the combined application of PBA and CsA could significantly enhance the beneficial effects, compared with using PBA or CsA alone.

Fri Feb 04 00:00:00 UTC 2022

Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA-328-3p-mediated inhibition of COL1A1.

Journal of cellular and molecular medicine

Liu T,Ye P,Ye Y,Lu S,Han B

Published figure using GAPDH monoclonal antibody (Product # A21994) in Western Blot

Sat Feb 01 00:00:00 UTC 2020

Long non-coding RNA LINC00968 reduces cell proliferation and migration and angiogenesis in breast cancer through up-regulation of PROX1 by reducing hsa-miR-423-5p.

Cell cycle (Georgetown, Tex.)

Sun X,Huang T,Zhang C,Zhang S,Wang Y,Zhang Q,Liu Z

A21994 was used in Western Blotting to determine the effect of LINC00968 in breast cancer (BC) and its underlying mechanism.

Thu Aug 01 00:00:00 UTC 2019

Functional analysis of the secondary HIV-1 capsid binding site in the host protein cyclophilin A.

Retrovirology

Peng W,Shi J,Márquez CL,Lau D,Walsh J,Faysal KMR,Byeon CH,Byeon IL,Aiken C,Böcking T

A21994 was used in Western Blotting to analyse the mechanism of the secondary HIV-1 capsid binding site in the host protein cyclophilin A.

Thu Apr 04 00:00:00 UTC 2019

The suppressive effects of miR-508-5p on the odontogenic differentiation of human dental pulp stem cells by targeting glycoprotein non-metastatic melanomal protein B.

Stem cell research & therapy

Liu F,Wang X,Yang Y,Hu R,Wang W,Wang Y

A21994 was used in Western Blotting to investigate the effects of miR-508-5p on the glycoprotein non-metastatic melanomal protein B expression and the odontogenic differentiation of human dental pulp stem cells.

Tue Jan 22 00:00:00 UTC 2019

microRNA-301b-3p downregulation underlies a novel inhibitory role of long non-coding RNA MBNL1-AS1 in non-small cell lung cancer.

Stem cell research & therapy

Li P,Xing W,Xu J,Yuan D,Liang G,Liu B,Ma H

A21994 was used in Western Blotting to determine the fucntional role of lncRNA MBNL1 in non-small cell lung cancer.

Tue May 21 00:00:00 UTC 2019

HIV-1 Engages a Dynein-Dynactin-BICD2 Complex for Infection and Transport to the Nucleus.

Journal of virology

Carnes SK,Zhou J,Aiken C

A21994 was used in Western Blotting to study whether HIV-1 engages the dynein motor complex via an adaptor for intracellular transport.

Mon Oct 15 00:00:00 UTC 2018

Long non-coding RNA MIF-AS1 promotes gastric cancer cell proliferation and reduces apoptosis to upregulate NDUFA4.

Cancer science

Li L,Li Y,Huang Y,Ouyang Y,Zhu Y,Wang Y,Guo X,Yuan Y,Gong K

A21994 was used in Western Blotting to determine the role of the long non-coding RNA MIF-AS1-based signal transduction pathway in mediating the proliferation and apoptosis of gastric cancer cells.

Sat Dec 01 00:00:00 UTC 2018

The δ-opioid receptor affects epidermal homeostasis via ERK-dependent inhibition of transcription factor POU2F3.

The Journal of investigative dermatology

Neumann C,Bigliardi-Qi M,Widmann C,Bigliardi PL

A21994 was used in western blot to investigate the role of delta-opioid receptor in human keratinocytes

Sun Feb 01 00:00:00 UTC 2015

生物信息学

蛋白别名: aging-associated gene 9 protein; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

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基因别名: CDABP0047; G3PD; GAPD; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12

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UniProt ID: (Human) P04406, (Human) O14556

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Entrez Gene ID: (Human) 2597, (Human) 26330

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功能

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding dehydrogenase

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