Invitrogen

GATA1 Recombinant Rabbit Monoclonal Antibody (4H15L5)

Advanced Verification
1 Reference
View all (30) GATA1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

700727

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

4H15L5

Immunogen

Recombinant protein corresponding to amino acids 1-200 of human GATA1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532341

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Eryf1; Erythroid transcription factor; erythroid transcription factor 1; GATA binding protein 1 (globin transcription factor 1); GATA-1; GATA-binding factor 1; GATA-binding protein 1; globin transcription factor 1; HGNC:4170; NF-E1 DNA-binding protein; nuclear factor, erythroid 1; transcription factor GATA1

View more View less

Gene Aliases: ERYF1; GATA-1; GATA1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT

View more View less

UniProt ID: (Human) P15976, (Mouse) P17679

View more View less

Entrez Gene ID: (Human) 2623, (Mouse) 14460

View more View less

Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding DNA binding, bending chromatin DNA binding sequence-specific DNA binding C2H2 zinc finger domain binding RNA polymerase II transcription factor activity, sequence-specific DNA binding chromatin binding metal ion binding DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development transcription from RNA polymerase II promoter cell-cell signaling heart development blood coagulation negative regulation of cell proliferation male gonad development organ morphogenesis tissue development regulation of glycoprotein biosynthetic process regulation of definitive erythrocyte differentiation regulation of primitive erythrocyte differentiation erythrocyte differentiation megakaryocyte differentiation platelet formation basophil differentiation eosinophil differentiation negative regulation of bone mineralization positive regulation of osteoblast proliferation embryonic hemopoiesis eosinophil fate commitment negative regulation of apoptotic process positive regulation of erythrocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract development erythrocyte development homeostasis of number of cells within a tissue positive regulation of peptidyl-tyrosine phosphorylation platelet aggregation transcriptional activation by promoter-enhancer looping dendritic cell differentiation cellular response to thyroid hormone stimulus negative regulation of transcription regulatory region DNA binding negative regulation of extrinsic apoptotic signaling pathway in absence of ligand transcription, DNA-templated regulation of transcription, DNA-templated myeloid cell differentiation cell development
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support