Bethyl Laboratories

GATA4 Polyclonal Antibody

查看其他19个GATA4抗体

抗体检测数据 (3)

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GATA4 Antibody (A303-503A) in IHC (P)

Detection of human GATA4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-GATA4 (Product # A303-503A) (Lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA4 Antibody in Western Blot (WB)

GATA4 Antibody in Immunoprecipitation (IP)

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GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Detection of human GATA4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-GATA4 (Product # A303-503A) (Lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

GATA4 Antibody in Western Blot (WB) — Detection of human GATA4 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, SK-N-MC, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GATA4 antibody (Product # A303-503A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

GATA4 Antibody in Immunoprecipitation (IP) — Detection of human GATA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GATA4 antibody (Product # A303-503A) (lot 2) used for IP at 6 µg per reaction. GATA4 was also immunoprecipitated by a previous lot of this antibody (Product # A303-503A) (lot 1) and a second antibody against a different epitope of GATA4 (BL12492). For blotting immunoprecipitated GATA4, A303-503A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

产品信息

A303-503A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000-1:25,000

-

免疫组化（石蜡） (IHC (P))

1:100 to 1:500

-

免疫沉淀 (IP)

2-10 µg/mg lysate

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 392 and 442 of human GATA Binding Protein 4.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

tris citrate/phosphate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat

靶标信息

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: GAT4; GATA-binding factor 4; MGC126629; Transcription factor GATA-4

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基因别名: ASD2; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694

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Entrez Gene ID: (Human) 2626

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RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding zinc ion binding protein kinase binding activating transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding NFAT protein binding co-SMAD binding DNA-binding transcription factor

heart looping endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell-cell signaling endoderm development blood coagulation male gonad development response to mechanical stimulus negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification response to drug cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development embryonic foregut morphogenesis anatomical structure formation involved in morphogenesis positive regulation of cardioblast differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction