Invitrogen

GATA6 Polyclonal Antibody

GATA6 Antibody in Immunocytochemistry (ICC/IF)

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GATA6 Antibody (PA1-104) in ICC/IF

Immunofluorescent analysis of GATA6 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a rabbit polyclonal antibody recognizing GATA6 (Product # PA1-104) at a dilution of 1:200 for at least 1 hour at 37°C. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:200 for 30 ... View More

GATA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA6 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GATA6 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot of GATA6 using anti-GATA6 polyclonal antibody (Product # PA1-104) shows increase in expression of GATA6 upon treatment with 5FU or PTX. {TM}

产品信息

PA1-104

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫细胞化学 (ICC/IF)

1:200

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence KYSGQDGLYIGVSLASPAE & KRFGAAGADASDSRAFPARE

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2539879

靶标信息

GATA6 is thought to be important for regulating terminal differentiation and/or proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Epigenetic regulation during cancer transitions across 11 tumour types.

Nature

Terekhanova NV,Karpova A,Liang WW,Strzalkowski A,Chen S,Li Y,Southard-Smith AN,Iglesia MD,Wendl MC,Jayasinghe RG,Liu J,Song Y,Cao S,Houston A,Liu X,Wyczalkowski MA,Lu RJ,Caravan W,Shinkle A,Naser Al Deen N,Herndon JM,Mudd J,Ma C,Sarkar H,Sato K,Ibrahim OM,Mo CK,Chasnoff SE,Porta-Pardo E,Held JM,Pachynski R,Schwarz JK,Gillanders WE,Kim AH,Vij R,DiPersio JF,Puram SV,Chheda MG,Fuh KC,DeNardo DG,Fields RC,Chen F,Raphael BJ,Ding L

PA1-104 was used in Immunohistochemistry to reveal a marked correlation between enhancer accessibility and gene expression and uncovered cooperation between epigenetic and genetic drivers.

Wed Nov 01 00:00:00 UTC 2023

Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre.

Nature communications

Xu PF,Borges RM,Fillatre J,de Oliveira-Melo M,Cheng T,Thisse B,Thisse C

PA1-104 was used in Immunohistochemistry to show that embryoids provide an in vitro model of mammalian embryo that displays extensive development of germ layer derivatives and that promises to be a powerful tool for in vitro studies and disease modelling.

Wed Jun 02 00:00:00 UTC 2021

Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre.

Nature communications

Xu PF,Borges RM,Fillatre J,de Oliveira-Melo M,Cheng T,Thisse B,Thisse C

Wed Jun 02 00:00:00 UTC 2021

Reproducible differentiation and characterization of neurons from mouse embryonic stem cells.

MethodsX

Saxena S,Choudhury S,Mohan KN

PA1-104 was used in Immunocytochemistry-immunoflourescence to describe a 16-day differentiation protocol that consistently induced differentiation of mESCs into neurons.