Invitrogen

GBF1 Polyclonal Antibody

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GBF1 Antibody (PA5-120173) in WB

Western blot analysis of GBF1 in extracts of HeLa cells. Samples were incubated with GBF1 Polyclonal antibody (Product # PA5-120173) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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产品信息

PA5-120173

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human GBF1 (NP_004184.1).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.6 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2913745

产品详细信息

Positive test controls include: HeLa. The target is usually found in the following locations: Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Lipid droplet, Membrane, Peripheral membrane protein, cis-Golgi network, trans-Golgi network.

Immunogen sequence: MVDKNIYIIQ GEINIVVGAI KRNARWSTHT PLDEERDPLL HSFGHLKEVL NSITELSEIE PNVFLRPFLE VIRSEDTTGP ITGLA

靶标信息

This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。