Invitrogen

GBL Polyclonal Antibody

GBL Antibody in Immunocytochemistry (ICC/IF)

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GBL Antibody (PA5-78558) in ICC/IF

Immunofluorescence analysis of GBL was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GBL Polyclonal Antibody (Product # PA5-78558) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution ... View More

GBL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GBL Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with GBL siRNA and reduction of signal was observed in Western Blot using GBL Polyclonal Antibody (Product # PA5-78558). {KD}

产品信息

PA5-78558

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Rat, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human mLST8/GBL. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.99 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2735825

产品详细信息

Positive Control: 293T, A431, HeLa, HepG2

Predicted Reactivity: Zebrafish (91%), Xenopus laevis (90%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

G-protein beta subunit like (GBL protein) is a novel member of WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of mTOR signaling complex (TORC1) and positively up-regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and also in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports suggest up-regulated expression of LST8 due to insulin indicating a possible role of LST8 in intracellular events related to insulin-receptor activation including insulin-stimulated glucose transport and glycogen synthesis. Northern Blot analysis detected a ubiquitous expression of LST8 with highest expression in brain and testis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: G beta-like protein; G protein beta subunit-like; Gable; GBL protein; Mammalian lethal with SEC13 protein 8; mLST8; mLST8 / mLST8; Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit gable; TORC subunit LST8; TORC subunit WAT1; transducin (beta)-like 4

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基因别名: 0610033N12Rik; AA409454; AI505104; AI851821; GbetaL; GBL; LST8; MLST8; POP3; WAT1

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UniProt ID: (Human) Q9BVC4, (Mouse) Q9DCJ1, (Rat) Q9Z2K5

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Entrez Gene ID: (Human) 64223, (Mouse) 56716, (Rat) 64226

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功能

protein binding molecular_function

参与通路