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Product Details
PA5-83330
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human GCLC. Recombinant protein control fragment (Product #RP-94586).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2790486
Product Specific Information
Immunogen sequence: FENSAYVVFV VLLTRVILSY KLDFLIPLSK VDENMKVAQK RDAVLQGMFY FRKDICKGGN AVVDGCGKAQ NSTELAAEEY TLMSIDTIIN GKEGVFPGLI P
Target Information
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Gamma-ECS; Gamma-glutamylcysteine synthetase; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit
Gene Aliases: GCL; GCLC; GCS; GLCL; GLCLC
UniProt ID: (Human) P48506
Entrez Gene ID: (Human) 2729
sulfur amino acid metabolic process
cysteine metabolic process
glutamate metabolic process
glutathione biosynthetic process
response to oxidative stress
apoptotic mitochondrial changes
response to heat
response to xenobiotic stimulus
response to hormone
L-ascorbic acid metabolic process
negative regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
cell redox homeostasis
negative regulation of transcription, DNA-templated
response to arsenic-containing substance
regulation of blood vessel size
response to nitrosative stress
regulation of mitochondrial depolarization
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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