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GCLC Antibody (MA5-35522) in IHC (P)
Immunohistochemistry analysis of GCLC in paraffin-embedded rat kidney. Samples were incubated with GCLC Monoclonal antibody (Product # MA5-35522) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
产品信息
MA5-35522
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC1065
抗原
A synthetic peptide corresponding to a sequence within amino acids 538-637 of human GCLC (P48506)
偶联物
形式
Liquid
浓度
1.7 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2849422
产品详细信息
Immunogen sequence: LNSYLENMEV DVDTRCSILN YLKLIKKRAS GELMTVARWM REFIANHPDY KQDSVITDEM NYSLILKCNQ IANELCECPE LLGSAFRKVK YSGSKTDSSN
靶标信息
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Gamma-ECS; Gamma-glutamylcysteine synthetase; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; Glutamylcysteine gamma synthetase light chain
基因别名: Gclc; Glclc
UniProt ID: (Rat) P19468
Entrez Gene ID: (Rat) 25283
cysteine metabolic process
glutamate metabolic process
glutathione metabolic process
glutathione biosynthetic process
response to oxidative stress
apoptotic mitochondrial changes
response to heat
response to xenobiotic stimulus
response to hormone
L-ascorbic acid metabolic process
negative regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
cell redox homeostasis
negative regulation of transcription, DNA-templated
response to arsenic-containing substance
regulation of blood vessel size
response to nitrosative stress
regulation of mitochondrial depolarization
negative regulation of extrinsic apoptotic signaling pathway
