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GCP2 Antibody (PA5-103851) in ICC/IF
Immunofluorescent analysis of GCP2 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GCP2 polyclonal antibody (Product # PA5-103851) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-103851
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TUBGCP2(Accession Q9BSJ2), corresponding to amino acid residues R540-L590.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2853183
产品详细信息
Antibody detects endogenous levels of total TUBGCP2.
靶标信息
The γ-Tubulin complex is composed of γ Tubulin and the γ-Tubulin complexassociated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. γ-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the γ-Tubulin complex. GCP2 (γ-Tubulin complex component 2), also known as TUBGCP2 or SPBC97 (spindle pole body protein Spc97 homolog) is a ubiquitously expressed 902 amino acid protein that localizes to the centrosome and is involved in microtubule nucleation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: a disintegrin and metalloprotease domain 8; Gamma-ring complex protein 103 kDa; Gamma-tubulin complex component 2; GCP-2; h103p; hGrip103; hSpc97; Spindle pole body protein Spc97 homolog; tubulin, gamma complex associated protein 2
基因别名: 1700022B05Rik; Adam8; GCP-2; GCP2; Grip103; h103p; hGCP2; hSpc97; SPBC97; Spc97p; TUBGCP2
UniProt ID: (Human) Q9BSJ2, (Mouse) Q921G8
Entrez Gene ID: (Human) 10844, (Mouse) 74237, (Rat) 309098
protein complex assembly
microtubule nucleation
meiotic nuclear division
cytoplasmic microtubule organization
centrosome duplication
interphase microtubule nucleation by interphase microtubule organizing center
mitotic spindle assembly
microtubule cytoskeleton organization
biological_process
proteolysis
integrin-mediated signaling pathway
