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GDF5 Antibody (PA5-47953) in IHC (F)
Immunohistochemical analysis of GDF5 in immersion fixed frozen sections of mouse embyro (12 d.p.c.). Samples were incubated in GDF5 polyclonal antibody (Product # PA5-47953) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
产品信息
PA5-47953
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant mouse GDF-5 Ala376-Arg495
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2608779
产品详细信息
In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) GDF-6 is observed, and less than 5% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF-8, rmGDF-9, recombinant human GDF-11, and rhGDF-15 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Hunter-Thompson type dwarfism and in Grebe Syndrome, which is characterized by short stature, extra digits, and short and deformed extremities. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino acid signal peptide and a 354 amino acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BMP-14; Bone morphogenetic protein 14; brachypodism; cartilage-derived morphogenetic protein-1; GDF-5; Growth/differentiation factor 5; H-GDF-5
基因别名: BMP-14; Bmp14; Bp; brp; Cdmp-1; Gdf-5; Gdf5
UniProt ID: (Mouse) P43027
Entrez Gene ID: (Mouse) 14563
chondrocyte differentiation
transmembrane receptor protein serine/threonine kinase signaling pathway
SMAD protein import into nucleus
positive regulation of pathway-restricted SMAD protein phosphorylation
embryonic limb morphogenesis
BMP signaling pathway
positive regulation of BMP signaling pathway
negative regulation of chondrocyte differentiation
positive regulation of chondrocyte differentiation
forelimb morphogenesis
hindlimb morphogenesis
growth
regulation of multicellular organism growth
regulation of MAPK cascade
negative regulation of neuron apoptotic process
positive regulation of neuron differentiation
cell development
negative regulation of epithelial cell proliferation
cartilage development
SMAD protein signal transduction
chondroblast differentiation
mitophagy in response to mitochondrial depolarization
negative regulation of mesenchymal cell apoptotic process
