Invitrogen

GDNF Polyclonal Antibody, Biotin, PeproTech®

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GDNF Antibody (500-P81BT-25UG) in WB

Western Blot: To detect hGDNF by Western Blot analysis GDNF Polyclonal Antibody, Biotin (Product # 500-P81BT-1MG) can be used at a concentration of 0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P81BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 mg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human GDNF

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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930783

产品详细信息

AA Sequence of recombinant protein: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDNF. Anti-Human GDNF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hGDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GDNF (500-P81) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGDNF.

Western Blot: To detect hGDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P81BT-1MG will be provided as 2 x 500 µg

靶标信息

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

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