Invitrogen

GFAP Polyclonal Antibody

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GFAP Antibody (PA1-9565) in WB

Western blot was performed using Anti-GFAP Rabbit Polyclonal Antibody (Product # PA1-9565) and a 50 kDa band corresponding to GFAP was observed across tissues tested except Mouse and Rat liver. Whole cell extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse liver (Lane 3) and Rat liver (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001)... View More

GFAP Antibody in Immunocytochemistry (ICC/IF)

GFAP Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

GFAP Antibody in Immunohistochemistry (IHC)

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GFAP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of GFAP was observed in Mouse brain, Rat brain in comparison to Mouse liver and Rat liver using Anti-GFAP Rabbit Polyclonal Antibody (Product # PA1-9565) in Western Blot. {RE}

产品信息

PA1-9565

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

免疫组化 (IHC)

1:500-1:1,000

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:500-1:1,000

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Human GFAP fusion protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2109797

产品详细信息

PA1-9565 detects GFAP in human, mouse and rat samples.

PA1-9565 has been successfully used in Western blot, immunohistochemistry, and immunocytochemistry procedures.

The PA1-9565 immunogen is a human GFAP fusion protein.

靶标信息

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Microfluidic Multielectrode Arrays for Spatially Localized Drug Delivery and Electrical Recordings of Primary Neuronal Cultures.

Frontiers in bioengineering and biotechnology

Bruno G,Colistra N,Melle G,Cerea A,Hubarevich A,Deleye L,De Angelis F,Dipalo M

PA1-9565 was used in Immunocytochemistry-Immunofluorescence to develop a microfluidic multielectrode array platform for performing electrical recordings of primary neurons and targeted drug delivery.

Mon Sep 28 00:00:00 UTC 2020

Microfluidic Multielectrode Arrays for Spatially Localized Drug Delivery and Electrical Recordings of Primary Neuronal Cultures.

Frontiers in bioengineering and biotechnology

Bruno G,Colistra N,Melle G,Cerea A,Hubarevich A,Deleye L,De Angelis F,Dipalo M

PA1-9565 was used in Immunocytochemistry-Immunofluorescence to develop a microfluidic multielectrode array platform for performing electrical recordings of primary neurons and targeted drug delivery.

Mon Sep 28 00:00:00 UTC 2020

Excessive Astrocytic GABA Causes Cortical Hypometabolism and Impedes Functional Recovery after Subcortical Stroke.

Cell reports

Nam MH,Cho J,Kwon DH,Park JY,Woo J,Lee JM,Lee S,Ko HY,Won W,Kim RG,Song H,Oh SJ,Choi JW,Park KD,Park EK,Jung H,Kim HS,Lee MC,Yun M,Lee CJ,Kim HI

PA1-9565 was used in Immunohistochemistry to show that in an animal model of internal capsular infarct, the GABA-synthesizing reactive astrocytes in distant cortical areas cause glucose hypometabolism via tonic inhibition of neighboring neurons.

Tue Jul 07 00:00:00 UTC 2020

Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas.

The Journal of comparative neurology

Akopian A,Kumar S,Ramakrishnan H,Viswanathan S,Bloomfield SA

PA1-9565 was used in immunohistochemistry - frozen section to examine the structural and functional integrity of amacrine cells in glaucoma samples

Tue Jan 01 00:00:00 UTC 2019

Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas.

The Journal of comparative neurology

Akopian A,Kumar S,Ramakrishnan H,Viswanathan S,Bloomfield SA

PA1-9565 was used in immunohistochemistry - frozen section to examine the structural and functional integrity of amacrine cells in glaucoma samples

Tue Jan 01 00:00:00 UTC 2019

Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas.

The Journal of comparative neurology

Akopian A,Kumar S,Ramakrishnan H,Viswanathan S,Bloomfield SA

PA1-9565 was used in immunohistochemistry - frozen section to examine the structural and functional integrity of amacrine cells in glaucoma samples

Tue Jan 01 00:00:00 UTC 2019

Acta histochemica

Yassa HD

PA1-9565 was used in immunohistochemistry - paraffin section to study age-related changes to the optic nerve using rats

Tue Jul 01 00:00:00 UTC 2014

Fasudil protects hippocampal neurons against hypoxia-reoxygenation injury by suppressing microglial inflammatory responses in mice.

Journal of neurochemistry

Ding J,Li QY,Wang X,Sun CH,Lu CZ,Xiao BG

PA1-9565 was used in immunohistochemistry to study the mechanism by which a Rho kinase inhibitor protects hippocampal neurons against hypoxia-reoxygenation injury

Wed Sep 01 00:00:00 UTC 2010

生物信息学

蛋白别名: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

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基因别名: AI836096; ALXDRD; GFAP

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UniProt ID: (Human) E9PAX3, (Rat) P47819, (Mouse) P03995

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Entrez Gene ID: (Human) 2670, (Rat) 24387, (Mouse) 14580

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功能

glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding structural molecule activity

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