Invitrogen

GFAP Monoclonal Antibody (2.2B10), Alexa Fluor™ 488, eBioscience™

GFAP Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

GFAP Antibody (53-9792-82) in ICC/IF

Immunofluorescence analysis of GFAP Monoclonal Antibody, Alexa Fluor 488 (Product # 53-9792-82) on rat astrocytes. Rat primary cortical astrocytes were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 1% Triton X-100 for 30 minutes at 37C, and blocked with ICC/IHC Blocking Buffer, Low Protein (Product # 00-4953-54) for 1 hour at room temperature. The cells were labeled with 10 µg/mL of GFAP Monoclonal Antibody, Alexa Fluor 488 (Product # 53-9792-82) in blocking buffer (Panel a: green). Nuclei (Panel b: ... View More

GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

GFAP Antibody — Intracellular staining of human cells. As expected based on known expression patterns, GFAP clone 2.2B10 stains CD44+ human astrocytes (top) and does not stain human peripheral blood lymphocytes (bottom). Details: Human astrocytes or normal human peripheral blood cells were fixed and permeablilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD44 (clone IM7) and either GFAP (clone 2.2B10, right) or Rat IgG2a kappa Isotype Control (left). Viable cells were used for the astrocyte analysis and cells in the lymphocyte gate were used for the peripheral blood analysis. {RE}

产品信息

53-9792-82

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

10 µg/mL

免疫细胞化学 (ICC/IF)

Assay-dependent

流式细胞分析 (Flow)

0.25 µg/test

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

2.2B10

抗原

Enriched bovine glial filaments

查看免疫原

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2762821

产品详细信息

Description: This 2.2B10 monoclonal antibody recognizes human, mouse, rat, and bovine glial fibrillary acid protein (GFAP). The immunogen used to generate this antibody was enriched bovine glial filaments.

Applications Reported: This 2.2B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 2.2B10 antibody has been tested by intracellular staining followed by flow cytometric analysis of U-251 cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

靶标信息

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

In mouse chronic pancreatitis CD25⁺FOXP3⁺ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response.

Nature communications

Glaubitz J,Wilden A,Golchert J,Homuth G,Völker U,Bröker BM,Thiele T,Lerch MM,Mayerle J,Aghdassi AA,Weiss FU,Sendler M

Published figure using GFAP monoclonal antibody (Product # 53-9792-82) in Flow Cytometry

Wed Aug 03 00:00:00 UTC 2022

生物信息学

蛋白别名: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

查看更多收起

基因别名: AI836096; ALXDRD; GFAP