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GFPT1 Antibody (PA5-119759) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of GFPT1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with GFPT1 Polyclonal Antibody (Product # PA5-119759) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
产品信息
PA5-119759
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein within human GFPT1 aa 1-200
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2913331
产品详细信息
Positive Control: 293 cell lysate, rat testis tissue lysate, mouse liver tissue lysate, LOVO, HepG2, rat kidney tissue, human liver tissue, mouse skeletal muscle tissue.
Subcellular Location: cytosol, extracellular exosome.
靶标信息
This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: D-fructose-6-phosphate amidotransferase 1; GFAT 1; glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1; Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1; glutamine:fructose 6 phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; Hexosephosphate aminotransferase 1
基因别名: 2810423A18Rik; AI324119; AI449986; CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1; GFPT1L; MSLG
UniProt ID: (Human) Q06210, (Rat) P82808, (Mouse) Q99MJ4
Entrez Gene ID: (Human) 2673, (Rat) 297417, (Mouse) 14583
carbohydrate metabolic process
fructose 6-phosphate metabolic process
glucosamine biosynthetic process
UDP-N-acetylglucosamine metabolic process
UDP-N-acetylglucosamine biosynthetic process
energy reserve metabolic process
glutamine metabolic process
response to sucrose
cellular response to insulin stimulus
circadian regulation of gene expression
IRE1-mediated unfolded protein response
negative regulation of glycogen biosynthetic process
protein homotetramerization
carbohydrate biosynthetic process
response to insulin
metabolic process
rhythmic process
carbohydrate derivative biosynthetic process
