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GHSR Antibody (PA5-28752) in ICC/IF
Immunofluorescence analysis of GHSR was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GHSR Polyclonal Antibody (Product # PA5-28752) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034)... View More
产品信息
PA5-28752
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to a region within amino acids 1 and 45 of Human GHS-R1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2546228
产品详细信息
Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, mouse hypothalamus, mouse brain, rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Growth hormone secretagogue receptor 1a (GHSR) is a member of the G-protein coupled receptor family. GHSR may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. Mutations to the gene can cause isolated partial growth hormone deficiency.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: G-protein coupled receptor; GH-releasing peptide receptor; Ghrelin receptor; GHRP; Ghrp receptor; GHS receptor; GHS-R; Growth hormone secretagogue receptor type 1
基因别名: C530020I22Rik; GHDP; GHSR
UniProt ID: (Human) Q92847, (Mouse) Q99P50
Entrez Gene ID: (Human) 2693, (Mouse) 208188
G-protein coupled receptor signaling pathway
actin polymerization or depolymerization
adult feeding behavior
response to hormone
hormone-mediated signaling pathway
growth hormone secretion
response to food
positive regulation of appetite
negative regulation of interleukin-1 beta production
cellular response to insulin stimulus
positive regulation of multicellular organism growth
negative regulation of tumor necrosis factor biosynthetic process
regulation of hindgut contraction
positive regulation of insulin-like growth factor receptor signaling pathway
negative regulation of interleukin-6 biosynthetic process
positive regulation of fatty acid metabolic process
negative regulation of insulin secretion
decidualization
negative regulation of inflammatory response
regulation of synapse assembly
signal transduction
