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GILZ Antibody (12-4033-82) in Flow
C57BL/6 lymph node cells were cultured for 3 hr with (purple histogram) or without (blue histogram) dexamethasone, followed by intracellular staining with 0.25 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (filled histogram) or 0.25 µg of Anti-Human/Mouse GILZ PE (filled histograms). CD3+ cells in the lymphocyte gate were used for analysis.
产品信息
12-4033-82
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
CFMKG15
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1659716
产品详细信息
Description: GILZ is a leucine-zipper-containing protein that is induced by glucocorticoids. It is expressed in mast cells, monocytes, macrophages, dendritic cells, and T cells. GILZ has been shown to inhibit production of IL-2 as well as TCR-driven upregulation of FasL. GILZ mediates these changes in transcription, at least in part, by inhibiting DNA-binding of the transcription factors AP-1 and NF-kappaB. GILZ has also been shown to mediate anti-inflammatory signals in macrophages and dendritic cells by interfering with NF-kappaB-mediated signaling. Although GILZ has been reported to localize to the nucleus, more recent reports as well as testing at eBioscience have observed predominantly cytoplasmic staining.
Applications Reported: This CFMKG15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.
Applications Tested: This CFMKG15 antibody has been tested by intracellular flow cytometric analysis of dexamethasone-treated mouse lymph node cells and human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For intracellular staining with CFMKG15, it is recommended to use IC Fixation Buffer (Product # 00-8222-49) followed by permeabilization with a 1X dilution of the 10X Permeabilization Buffer (Product # 00-8333-56).
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The TSC22 domain family member 3 protein (TSC22D3) is a leucine zipper protein that functions as a transcriptional regulator. The expression of TSC22D3 is stimulated by glucocorticoids and IL-10 and is thought to play a key role in the anti-inflammatory and immunosuppressive effects of these molecules. TSC22D3 can physically interact with and inhibit the activities of key inflammatory signaling mediators such NF-Kappa-B and AP-1. TSC22D3 functions as a transcriptional co-activator for various nuclear receptors and NF-Kappa-B. It has also been shown to be involved in the differentiation of mesenchymal stem cells towards osteoblasts and bone formation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: delta sleep inducing peptide, immunoreactor; Delta sleep-inducing peptide immunoreactor; DSIP-immunoreactive leucine zipper protein; DSIP-immunoreactive peptide; GILZ; Glucocorticoid-induced leucine zipper protein; long glucocorticoid-induced leucine zipper protein; Protein DIP; Tilz3; TSC-22 related protein; TSC-22-like protein; TSC-22-related protein; TSC-22R; TSC22 domain family protein 3; TSC22 domain family, member 3; TSC22-related inducible leucine zipper 3; TSC22-related-inducible leucine zipper 3
基因别名: DIP; Dsip1; DSIPI; GILZ; Tilz3; TSC-22R; TSC22D3
UniProt ID: (Human) Q99576, (Mouse) Q9Z2S7
Entrez Gene ID: (Human) 1831, (Mouse) 14605
