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GITRL Chimeric Antibody (MA5-48019) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of GITRL in mouse splenocytes using GITRL Chimeric Monoclonal Antibody (Product # MA5-48019). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with GITRL Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing membrane and some cytoplasmic staining in a subset of cells. The nuclea... View More
产品信息
MA5-48019
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
YGL386
抗原
Recombinant protein injected into DA rats.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.02% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3074462
产品详细信息
Specificity: This antibody binds murine GITRL.
靶标信息
The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-kappa-B and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells and can activate NF-kappa-B, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-kappa-B activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GITR ligand; GITRL; GITRLigand; Glucocorticoid-induced TNF-related ligand; glucocorticoid-induced-tumor necrosis factor receptor ligand; RP1-15D23.1; TNF superfamily member 18; Tumor necrosis factor ligand superfamily member 18
基因别名: Gitrl; Tnfsf18
UniProt ID: (Mouse) Q7TS55
Entrez Gene ID: (Mouse) 240873
T cell proliferation involved in immune response
immune system process
positive regulation of leukocyte migration
immune response
cell-cell signaling
positive regulation of macrophage chemotaxis
interleukin-10 production
interleukin-12 production
regulation of interferon-gamma production
regulation of interleukin-6 production
regulation of tumor necrosis factor production
tumor necrosis factor-mediated signaling pathway
activated CD4-positive, alpha-beta T cell proliferation
regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of cell adhesion
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
regulation of monocyte chemotactic protein-1 production
positive regulation of monocyte chemotaxis
positive regulation of protein homodimerization activity
negative regulation of T-helper 17 cell lineage commitment
regulation of CD40 signaling pathway
regulation of dendritic cell chemotaxis
