Invitrogen

GLI2 Polyclonal Antibody

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抗体检测数据 (1)

GLI2 Antibody in Western Blot (WB)

GLI2 Antibody in Western Blot (WB)

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GLI2 Antibody (PA5-93021) in WB

Western blot analysis of GLI2 in extracts of various cell lines. Samples were incubated with GLI2 Polyclonal antibody (Product # PA5-93021) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

GLI2 Antibody in Western Blot (WB)

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GLI2 Antibody in Western Blot (WB) — Western blot analysis of GLI2 in extracts of various cell lines. Samples were incubated with GLI2 Polyclonal antibody (Product # PA5-93021) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

产品信息

PA5-93021

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of mus musculus GLI2 (NP_0010745941)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.23 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2806593

产品详细信息

Positive Samples: Mouse lung, Mouse brain, Mouse heart, Mouse ovary, Mouse pancreas, Rat brain; Cellular Location: Cytoplasm, Nucleus

Immunogen sequence: METSAPAPAL EKKEAKSGLL EDSSFPDPGK KACPLAVAAA VAAHGVPQQL LPAFHAPLPI DMRHQEGRYH YDPHSVHSVH GPPTLSGSPV ISDISLIRLS PHPAGPGESP FSAHHPYVNP HMEHYLRSVH SSPTLSMISA ARGLSPADVA HEHLKERGLF SLAAPGTNPS DYYHQ

靶标信息

Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: GLI-Kruppel family member GLI2; Tax helper protein; Zinc finger protein GLI2

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基因别名: AW546128; Gli2; Thp

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UniProt ID: (Mouse) Q0VGT2

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Entrez Gene ID: (Rat) 304729, (Mouse) 14633

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding molecular_function DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding zinc ion binding sequence-specific DNA binding transcription regulatory region DNA binding nucleic acid binding protein binding metal ion binding DNA-binding transcription factor

negative regulation of transcription from RNA polymerase II promoter skeletal system development osteoblast differentiation in utero embryonic development kidney development morphogenesis of an epithelium chondrocyte differentiation osteoblast development transcription from RNA polymerase II promoter smoothened signaling pathway pattern specification process axon guidance ventral midline development hindgut morphogenesis heart development cell proliferation positive regulation of cell proliferation regulation of smoothened signaling pathway response to mechanical stimulus epidermal cell differentiation dorsal/ventral pattern formation proximal/distal pattern formation floor plate formation spinal cord dorsal/ventral patterning ventral spinal cord development spinal cord motor neuron differentiation cerebellar cortex morphogenesis smoothened signaling pathway involved in ventral spinal cord interneuron specification smoothened signaling pathway involved in spinal cord motor neuron cell fate specification dorsal/ventral neural tube patterning neural tube development smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation spinal cord ventral commissure morphogenesis pituitary gland development cell differentiation lung development mammary gland development hindbrain development negative regulation of chondrocyte differentiation tube development odontogenesis of dentin-containing tooth embryonic digit morphogenesis positive regulation of neuron differentiation positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract development developmental growth anatomical structure formation involved in morphogenesis neuron development branching morphogenesis of an epithelial tube anatomical structure development notochord regression head development prostatic bud formation mammary gland duct morphogenesis smoothened signaling pathway involved in dorsal/ventral neural tube patterning cellular response to organic cyclic compound cochlea morphogenesis cellular response to virus transcription, DNA-templated regulation of transcription, DNA-templated