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Invitrogen
GLUT3 Recombinant Rabbit Monoclonal Antibody (JA50-31)
FIGURE: 1 / 4
GLUT3 Antibody (MA5-32697) in ICC/IF
Immunofluorescence analysis of GLUT3 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GLUT3 Rabbit Recombinant Monoclonal Antibody (JA50-31) (Product # MA5-32697) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pro... View More
Product Details
MA5-32697
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA50-31
Immunogen
Synthetic peptide within Human GLUT3 aa 11-60
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809974
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ90380; glucose transporter 3; Glucose transporter type 3, brain; GLUT-3; solute carrier family 2 (facilitated glucose transporter), member 3; Solute carrier family 2 A3 (neuron glucose transporter); Solute carrier family 2, facilitated glucose transporter member 3; solute carrier family 2, member 2; solute carrier family 2, member 3
Gene Aliases: AA408729; AL023014; AL024341; AU040424; C78366; Glut-3; GLUT3; SLC2A3
UniProt ID: (Human) P11169, (Mouse) P32037, (Rat) Q07647
Entrez Gene ID: (Human) 6515, (Mouse) 20527, (Rat) 25551
glucose transmembrane transporter activity
glucose binding
dehydroascorbic acid transporter activity
D-glucose transmembrane transporter activity
transporter activity
sugar:proton symporter activity
monosaccharide transmembrane transporter activity
galactoside binding
kinase binding
transmembrane transporter activity
substrate-specific transmembrane transporter activity
xylose binding
protein binding
transport
carbohydrate transport
glucose transport
proton transport
hexose transmembrane transport
glucose import
transmembrane transport
dehydroascorbic acid transport
cation transmembrane transport
carbohydrate metabolic process
L-ascorbic acid metabolic process
glucose transmembrane transport
response to glucose
monosaccharide transport
negative regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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