Invitrogen

GLUT4 Monoclonal Antibody (3G10A3)

GLUT4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

GLUT4 Antibody (MA5-17176) in ICC/IF

Immunofluorescence analysis of GLUT4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GLUT4 Monoclonal Antibody (3G10A3) (Product # MA5-17176, 1:200) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:... View More

GLUT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

MA5-17176

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

3G10A3

抗原

Purified recombinant fragment of human SLC2A4 (amino acids: 224-353 ) expressed in E. Coli.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538647

产品详细信息

MA5-17176 targets SLC2A4 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17176 immunogen is purified recombinant fragment of human SLC2A4 (amino acids: 224-353 ) expressed in E. Coli.

MA5-17176 detects SLC2A4 which has a predicted molecular weight of approximately 54.8kDa.

靶标信息

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Chemical systems biology reveals mechanisms of glucocorticoid receptor signaling.

Nature chemical biology

Bruno NE,Nwachukwu JC,Srinivasan S,Nettles CC,Izard T,Jin Z,Nowak J,Cameron MD,Boregowda SV,Phinney DG,Elemento O,Liu X,Ortlund EA,Houtman R,Stavreva DA,Hager GL,Kamenecka TM,Kojetin DJ,Nettles KW

MA5-17176 was used in Western Blot to examine ligands designed to modulate glucocorticoid receptor activity through distinct structural mechanisms.

Mon Mar 01 00:00:00 UTC 2021

D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes.

Frontiers in endocrinology

Montt-Guevara MM,Finiguerra M,Marzi I,Fidecicchi T,Ferrari A,Genazzani AD,Simoncini T

Published figure using GLUT4 monoclonal antibody (Product # MA5-17176) in Western Blot

Wed Jan 12 00:00:00 UTC 2022

Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluation.

Scientific reports

Slaughter VL,Rumsey JW,Boone R,Malik D,Cai Y,Sriram NN,Long CJ,McAleer CW,Lambert S,Shuler ML,Hickman JJ

MA5-17176 was used in Immunocytochemistry to develop and characterize a microfluidic HoaC model composed of adipocytes representing white adipose tissue and functional liver cell chambers in recirculating, serum-free medium to study aspects of nonalcoholic fatty liver disease.

Wed Jun 23 00:00:00 UTC 2021

Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluation.

Scientific reports

Slaughter VL,Rumsey JW,Boone R,Malik D,Cai Y,Sriram NN,Long CJ,McAleer CW,Lambert S,Shuler ML,Hickman JJ

Wed Jun 23 00:00:00 UTC 2021

Adipose Lipolysis Regulates Cardiac Glucose Uptake and Function in Mice under Cold Stress.

International journal of molecular sciences

Choi Y,Shin H,Tang Z,Yeh Y,Ma Y,Kadegowda AKG,Wang H,Jiang L,Arya RK,Chen L,Xue B,Shi H,Gavrilova O,Yu L

MA5-17176 was used in Western Blotting to show that mice with selective inactivation of the lipolytic coactivator comparative gene identification-58 (CGI-58) in adipose tissue (FAT-KO mice), relative to their littermate controls, had lower circulating FA levels in the fed and fasted states due to impaired adipose lipolysis.

Sun Dec 12 00:00:00 UTC 2021

Increased glucose metabolism in Arid5b^-/- skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1).

Biological research

Okazaki Y,Murray J,Ehsani A,Clark J,Whitson RH,Hirose L,Yanaka N,Itakura K

MA5-17176 was used in Immunocytochemistry to demonstrate previously that AT-rich interactive domain 5b (Arid5b) knockout (Arid5b-/-) mice were lean and resistant to high-fat diet (HFD)-induced obesity.

Tue Oct 06 00:00:00 UTC 2020

Muscle structural, energetic and functional benefits of endurance exercise training in sickle cell disease.

American journal of hematology

Merlet AN,Féasson L,Bartolucci P,Hourdé C,Schwalm C,Gellen B,Galactéros F,Deldicque L,Francaux M,Messonnier LA

MA5-17176 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that moderate-intensity endurance exercise improves exercise capacity and muscle structural and oxidative properties in patients with sickle cell disease.

Sun Nov 01 00:00:00 UTC 2020

Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Fernández-Verdejo R,Vanwynsberghe AM,Essaghir A,Demoulin JB,Hai T,Deldicque L,Francaux M

MA517176 was used in western blot to identify target genes of activating transcription factor 3 involved in muscle adaptation to training

Wed Feb 01 00:00:00 UTC 2017

生物信息学

蛋白别名: glucose transporter glut4; Glucose transporter type 4, insulin-responsive; GLUT-4; GT2; insulin-responsive glucose transporter type 4; MGC93607; solute carrier family 2 (facilitated glucose transporter), member 4; Solute carrier family 2, facilitated glucose transporter member 4

查看更多收起

基因别名: Glut-4; GLUT4; SLC2A4

查看更多收起

UniProt ID: (Human) P14672, (Mouse) P14142

查看更多收起

Entrez Gene ID: (Human) 6517, (Mouse) 20528

查看更多收起

功能

glucose transmembrane transporter activity protein binding D-glucose transmembrane transporter activity transporter activity insulin-responsive hydrogen:glucose symporter activity transmembrane transporter activity substrate-specific transmembrane transporter activity

参与通路