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GM-CSF Antibody (500-P33-1MG) in WB
Western Blot: To detect hGM-CSF by Western Blot analysis GM-CSF Polyclonal Antibody (Product # 500-P33-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P33-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human GM-CSF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930468
产品详细信息
AA Sequence of recombinant protein: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GM-CSF. Anti-Human GM-CSF-specific antibody was purified by affinity chromatography employing an immobilized Human GM-CSF matrix.
Sandwich ELISA: To detect Human GM-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GM-CSF (500-P33Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GM-CSF.
Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; Molgramostin; Sargramostim
基因别名: CSF2; GMCSF
UniProt ID: (Human) P04141
Entrez Gene ID: (Human) 1437
MAPK cascade
embryonic placenta development
immune response
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
peptidyl-tyrosine phosphorylation
positive regulation of interleukin-23 production
epithelial fluid transport
macrophage activation
positive regulation of tyrosine phosphorylation of Stat5 protein
myeloid dendritic cell differentiation
positive regulation of GTPase activity
positive regulation of DNA replication
negative regulation of cytolysis
cellular response to lipopolysaccharide
positive regulation of podosome assembly
dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
