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产品信息
MM500C
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
22E9
抗原
Recombinant mouse GM-CSF
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_223532
产品详细信息
MM500C targets Mouse GM-CSF in ELISA, and WB applications and shows reactivity with mouse samples.
The MM500C immunogen is recombinant mouse GM-CSF.
MM500C detects Mouse GM-CSF which has a predicted molecular weight of approximately 14 kDa.
The MM500C mouse GMCSF antibody (clone 22E9) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM500DB (biotinylated conjugate of clone 31G6) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody MM500C (clone 22E9) and biotinylated antibody MM500DB (clone 31G6) have successfully been used in combination with recombinant mouse GMCSF protein SMGMCSF in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
靶标信息
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; put. GM-CSF
基因别名: Csf2; Csfgm; Gm-CSf; GMCSF; MGI-IGM
UniProt ID: (Mouse) P01587
Entrez Gene ID: (Mouse) 12981
embryonic placenta development
immune response
positive regulation of cell proliferation
regulation of gene expression
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of interleukin-23 production
epithelial fluid transport
macrophage activation
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
myeloid dendritic cell differentiation
positive regulation of DNA replication
negative regulation of cytolysis
positive regulation of podosome assembly
dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
