Invitrogen

GNAI1/GNAI2 Polyclonal Antibody

GNAI1/GNAI2 Antibody in Immunocytochemistry (ICC/IF)

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GNAI1/GNAI2 Antibody (PA1-1000) in ICC/IF

Immunofluorescence analysis of G Protein alpha Inhibitor 1 & 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GNAI1/GNAI2 Rabbit Polyclonal Antibody (Product # PA1-1000) at 2 µg/mL in 0.1% BSA and i... View More

GNAI1/GNAI2 Antibody in Western Blot (WB)

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产品信息

PA1-1000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫细胞化学 (ICC/IF)

2 µg/mL

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues K(346) N N L K D C G L F(355) of human G Protein alpha Inhibitor 1/2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2232440

产品详细信息

PA1-1000 detects G protein alpha inhibitor 1/2 from human, rat, and mouse tissues and cells.

PA1-1000 has been successfully used in Immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~38 kDa protein representing G protein alpha inhibitor 1/2 from HeLa cell lysate.

The PA1-1000 immunogen is a synthetic peptide corresponding to residues K(346) N N L K D C G L F(355) of human G Protein alpha Inhibitor 1/2. This sequence is completely conserved in human, rat, guinea pig, hamster, bovine, hydra, octopus, Xenopus, chicken, canine, spiny dogfish, great pond snail, starfish, and American lobster G protein alpha inhibitor 1/2. PA1-1000 immunizing peptide (Cat. # PEP-129) is available for use in neutralization and control procedures.

靶标信息

The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Differential effects of antidepressants escitalopram versus lithium on Gs alpha membrane relocalization.

BMC neuroscience

Donati RJ,Schappi J,Czysz AH,Jackson A,Rasenick MM

Published figure using GNAI1/GNAI2 polyclonal antibody (Product # PA1-1000) in Western Blot

Sat Jul 11 00:00:00 UTC 2015

Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1.

Proceedings of the National Academy of Sciences of the United States of America

Jang HJ,Kokrashvili Z,Theodorakis MJ,Carlson OD,Kim BJ,Zhou J,Kim HH,Xu X,Chan SL,Juhaszova M,Bernier M,Mosinger B,Margolskee RF,Egan JM

PA1-1000 was used in immunoprecipitation to investigate the effects of gustducin and taste receptors on glucagon-like peptide-1

Tue Sep 18 00:00:00 UTC 2007

生物信息学

蛋白别名: Adenylate cyclase-inhibiting G alpha protein; G alpha inhibitory type 2 (a); G alpha inhibitory type 2 (b); Gi1 protein alpha subunit; GTP-binding protein (G-alpha-i2); GTP-binding regulatory protein Gi alpha-2 chain; guanine nucleotide binding protein (G protein), alpha inhibiting 1; guanine nucleotide binding protein (G protein), alpha inhibiting 2; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2; guanine nucleotide binding protein, alpha inhibiting 1; guanine nucleotide binding protein, alpha inhibiting 2; Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(i) subunit alpha-2; guanine nucleotide-binding protein G(i), alpha-1 subunit; guanine nucleotide-binding protein G(i), alpha-2 subunit

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基因别名: AU046200; BPGTPB; C76432; Galphai2; Gi; Gia; Gialpha1; GIP; Gnai-1; Gnai-2; GNAI1; GNAI2; GNAI2B; H_LUCA15.1; H_LUCA16.1