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GNAI3 Antibody (PA5-76885) in ICC/IF
Immunofluorescence analysis of GNAI3 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GNAI3 Polyclonal Antibody (Product # PA5-76885) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A2... View More
Product Details
PA5-76885
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human GNAI3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2720612
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i. e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Galpha subunits have been identified; these include Gs, Gi, Gq and Galpha 12/13. The Gi class comprises all the known alpha subunits that are susceptible to pertussis toxin modifications, including Galpha i-1, Galpha i-2, Galpha i-3, Galpha o, Galpha t1, Galpha t2, Galpha z and Galpha gust. Of these, the three Galpha i subtypes function to open atrial potassium channels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G(i) alpha-3; guanine nucleotide binding protein (G protein), alpha inhibiting 3; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3; guanine nucleotide binding protein, alpha inhibiting 3; Guanine nucleotide binding, protein, alpha inhibiting polypeptide 3; Guanine nucleotide-binding protein G(i) subunit alpha-3; guanine nucleotide-binding protein G(k) subunit alpha
Gene Aliases: 87U6; ARCND1; GIP3A; Gnai-3; GNAI3
UniProt ID: (Human) P08754, (Rat) P08753
Entrez Gene ID: (Human) 2773, (Rat) 25643
G-protein coupled receptor binding
GTPase activity
signal transducer activity
protein binding
GTP binding
GDP binding
protein domain specific binding
G-protein beta/gamma-subunit complex binding
G-protein coupled serotonin receptor binding
GTPase activating protein binding
metal ion binding
heterotrimeric G-protein
protein folding
transport
vesicle fusion
cell cycle
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
negative regulation of adenylate cyclase activity
dopamine receptor signaling pathway
GTP metabolic process
cell division
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
regulation of heart contraction
metabolic process
negative regulation of secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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