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GNG5 Antibody (PA5-103878) in ICC/IF
Immunocytochemistry analysis of GNG5 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GNG5 polyclonal antibody (Product # PA5-103878) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103878
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human GNG5(Accession P63218), corresponding to amino acid residues A32-L68.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2853210
产品详细信息
Antibody detects endogenous levels of total GNG5.
靶标信息
G proteins are trimeric (alpha-beta-gamma) membrane-associated proteins that regulate flow of information from cell surface receptors to a variety of internal metabolic effectors. Interaction of a G protein with its activated receptor promotes exchange of GTP for GDP that is bound to the alpha subunit. The alpha-GTP complex dissociates from the beta-gamma heterodimer so that the subunits, in turn, may interact with and regulate effector molecules (Gilman, 1987 [PubMed 3113327];Summary by Ahmad et al., 1995) [PubMed 7606925].[supplied by OMIM, Nov 2010].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: G protein gamma-5 subunit; guanine nucleotide binding protein (G protein), gamma 5; guanine nucleotide binding protein (G protein), gamma 5 subunit; guanine nucleotide binding protein gamma 5; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-5
基因别名: G(y)5; GNG5; GNGT5
UniProt ID: (Human) B2R5A0, (Mouse) Q80SZ7, (Rat) P63219
Entrez Gene ID: (Human) 2787, (Mouse) 14707, (Rat) 79218
