Invitrogen

GPBB Polyclonal Antibody

GPBB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GPBB Antibody (PA5-28022) in IHC (P)

GPBB Polyclonal Antibody detects GPBB protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. GPBB Polyclonal Antibody (Product # PA5-28022) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

GPBB Antibody — Glycogen phosphorylase brain isoform knockdown inhibits glycogen utilization in breast cancer cells. (A) Western blot of liver and brain glycogen phosphorylase (PYGL and PYGB) in wild-type, either empty vector, shPYGL and shPYGB transduced MDA-MB-231, MCF-7, and MCF-10A cells. Actin is shown as a loading control(B-D) Glycogen levels normalized to protein in lysate after 24h in hypoxia (Hypoxic Control) or 24h in hypoxia with subsequent 24h in normoxia (Normoxic Exposure) for wild-type, empty vector, shPYGL, and shPYGB in (B) MDA-MB-231, (C) MCF-7, and (D) MCF-10A cells, respectively. Error bars represent SEM of n = 3. *p<0.05, **p<0.01 compared to EV by multiple comparison one-way ANOVA. {KD}

产品信息

PA5-28022

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 519 and 813 of Human GPBB

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.36 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545498

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain.

Predicted reactivity: Mouse (92%), Rat (91%), Zebrafish (85%), Chicken (86%), Sheep (92%), Rhesus Monkey (98%), Bovine (92%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。