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GPCR/G-protein complex-stabilizing scFv Antibody (703976-1MG)
A tagged GPCR was used to pull down a GPCR-heterotrimeric G protein complex that is stabilized by scFv16 (Product # 703976). A) Size exclusion chromatography (SEC) chromatogram of the purified complex. B) Highlighted sample from SEC was run on SDS-PAGE gel and Coomassie stained. All components of the active complex are present. C) Direct visualization of GPCR-G protein complex by negative stain TEM showing features characteristic for active GPCR complexes that are intact. Data courtesy Dr. David M. Thal, Monash Institute of Pharmaceutical ... View More
产品信息
703976-1MG
产品规格
种属反应
Human
宿主/亚型
Mouse
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
scFv16
抗原
Rhodopsin/Gi1
偶联物
形式
Liquid
浓度
2.0 mg/mL
纯化类型
Affinity chromatography
保存液
25mM HEPES, pH 7.5, with 10% glycerol
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2896321
产品详细信息
The scFv antibody stabilizes GPCR/G-protein complexes for cryo-EM of active structures. It recognizes an interface between G alpha and G beta/gamma subunits, and confers resistance to nucleotide exchange-triggered dissociation of the heterotrimeric G-protein. In addition to members of the Gi/o family, this antibody fragment can also be applied to chimeric variants and stabilize other G-protein subtypes including Gs complexes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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