Invitrogen

GPI Polyclonal Antibody

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GPI Antibody (PA5-26787) in WB

Western blot analysis of GPI in Ramos cell line, mouse liver, rat liver tissue (from left to right). Samples were incubated with GPI polyclonal antibody (Product # PA5-26787) using a dilution of 1:1,000 followed by goat anti-rabbit IgG H&L (HRP) at a dilution of 1:5,000. Lysates at 35 µg per lane.

GPI Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

Product Image Gallery

GPI Antibody — Figure 1. N-glycan branching controls T H 17 versus iTreg cell fate. ( A ) Fructose 6-phosphate and glutamine may be metabolized by glycolysis and glutaminolysis, respectively, or enter the hexosamine pathway to supply UDP-GlcNAc to the Golgi branching enzymes Mgat1, 2, 4 and 5. HK: hexokinase, GPI: glucose-6-phosphate isomerase, PFK1: phosphofructokinase1, LDH: Lactate dehydrogenase, GFPT: glutamine-fructose-6-phosphate transaminase. ( B - H ) Flow cytometry ( B , E - H ), Western blot ( C ) and LC-MS/MS ( D ) analysis of purified mouse splenic CD4 + T-cells activated with anti-CD3+anti-CD28 for 4 days ( B , E - H ) or 3 days ( C,D ) with T H 17 inducing conditions (TGFbeta+IL-6+IL-23) or as indicated. PFK1-L (liver), PFK1-P (platelet), PFK1-M (muscle). ( G ) Co-incubation with doxycycline in vitro. ( H ) Doxycycline treatment in vivo, with Mgat1 f/f tetO-Cre + ROSA rtTA cells in right panel gated on L-PHA - population. ( B , E - H ) gated on CD4 + . ( B , D - H ) Unpaired two tailed t -test with Welch's ( E ) and Bonferroni corrections ( B , E ). **p<0.01; ***p<0.001. Data are mean +- s.e.m of triplicate cultures and representative of n >= 3 experiments. MFI, mean fluorescence intensity. DOI: http://dx.doi.org/ Figure 1--figure supplement 1. N-glycan branching controls T H 17 versus iTreg cell fate. ( A-F ) Flow cytometry ( A,B,E,F ), real-time qPCR ( C ) and UDP-GlcNAc LC-MS/MS analysis ( D ) of purified mouse splenic CD4 + T-cells activated with anti-CD3+anti-CD28 under T {TM}

产品信息

PA5-26787

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

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流式细胞分析 (Flow)

1:10-1:50

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human GPI

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2544287

产品详细信息

This antibody is predicted to react with non-human primate based on sequence homology.

靶标信息

This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Huntington disease oligodendrocyte maturation deficits revealed by single-nucleus RNAseq are rescued by thiamine-biotin supplementation.

Nature communications

Lim RG,Al-Dalahmah O,Wu J,Gold MP,Reidling JC,Tang G,Adam M,Dansu DK,Park HJ,Casaccia P,Miramontes R,Reyes-Ortiz AM,Lau A,Hickman RA,Khan F,Paryani F,Tang A,Ofori K,Miyoshi E,Michael N,McClure N,Flowers XE,Vonsattel JP,Davidson S,Menon V,Swarup V,Fraenkel E,Goldman JE,Thompson LM

PA5-26787 was used in Western Blotting to identify cell type-specific and -agnostic signatures suggesting oligodendrocytes (OLs) and oligodendrocyte precursors (OPCs) are arrested in intermediate maturation states.

Wed Dec 21 00:00:00 UTC 2022

Type I interferon decreases macrophage energy metabolism during mycobacterial infection.

Cell reports

Olson GS,Murray TA,Jahn AN,Mai D,Diercks AH,Gold ES,Aderem A

PA5-26787 was used in Flow Cytometry to suggest that a type I IFN-mitochondrial feedback loop controls macrophage responses to mycobacteria and that this could contribute to pathogenesis across a range of diseases.

Tue Jun 01 00:00:00 UTC 2021

Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.

Cell

Wu L,Hollinshead KER,Hao Y,Au C,Kroehling L,Ng C,Lin WY,Li D,Silva HM,Shin J,Lafaille JJ,Possemato R,Pacold ME,Papagiannakopoulos T,Kimmelman AC,Satija R,Littman DR

PA5-26787 was used in Western Blot to suggest that inhibiting glycolysis by targeting Gpi1 could be an effective therapeutic strategy with minimum toxicity for Th17-mediated autoimmune diseases, and, more generally, that metabolic redundancies can be exploited for selective targeting of disease processes.

Thu Aug 06 00:00:00 UTC 2020

Glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation.

eLife

Araujo L,Khim P,Mkhikian H,Mortales CL,Demetriou M

PA526787 was used in western blot to assess if aerobic glycolysis and glutaminolysis co-operatively reduce UDP-GlcNAc biosynthesis and N-glycan branching in mouse T cell blasts

Fri Jan 06 00:00:00 UTC 2017

Stable Isotope Labeling with Amino Acids (SILAC)-Based Proteomics of Primary Human Kidney Cells Reveals a Novel Link between Male Sex Hormones and Impaired Energy Metabolism in Diabetic Kidney Disease.

Molecular & cellular proteomics : MCP

Clotet S,Soler MJ,Riera M,Pascual J,Fang F,Zhou J,Batruch I,Vasiliou SK,Dimitromanolakis A,Barrios C,Diamandis EP,Scholey JW,Konvalinka A

PA526787 was used in immunohistochemistry - paraffin section and western blot to use stable isotope labeling with amino acids to measure the proteome in dihydrotestosterone- and 17beta-estradiol-treated human proximal tubular epithelial cells

Wed Mar 01 00:00:00 UTC 2017

Molecular & cellular proteomics : MCP

Clotet S,Soler MJ,Riera M,Pascual J,Fang F,Zhou J,Batruch I,Vasiliou SK,Dimitromanolakis A,Barrios C,Diamandis EP,Scholey JW,Konvalinka A

Wed Mar 01 00:00:00 UTC 2017

Molecular & cellular proteomics : MCP

Clotet S,Soler MJ,Riera M,Pascual J,Fang F,Zhou J,Batruch I,Vasiliou SK,Dimitromanolakis A,Barrios C,Diamandis EP,Scholey JW,Konvalinka A

Wed Mar 01 00:00:00 UTC 2017

生物信息学

蛋白别名: AMF; Autocrine motility factor; glucose phosphate isomerase; glucose phosphate isomerase 1 complex; glucose phosphate isomerase 1, regulatory; glucose phosphate isomerase 1, structural; glucose phosphate isomerase 1, temporal; Glucose-6-phosphate isomerase; GPI; hexose monophosphate isomerase; hexosephosphate isomerase; maturation factor; Neuroleukin; NLK; oxoisomerase; PGI; PHI; Phosphoglucose isomerase; phosphohexomutase; Phosphohexose isomerase; phosphosaccharomutase; SA-36; sperm antigen; Sperm antigen 36; sperm antigen-36

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基因别名: AMF; GNPI; GPI; Gpi-1; Gpi-1r; Gpi-1s; Gpi-1t; Gpi1; Gpi1-r; Gpi1-s; Gpi1-t; Gpi1s; MF; NK; NK/GPI; NLK; Org; PGI; PHI; SA-36; SA36

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UniProt ID: (Human) P06744, (Mouse) P06745

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Entrez Gene ID: (Human) 2821, (Mouse) 14751

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功能

glucose-6-phosphate isomerase activity cytokine activity growth factor activity intramolecular transferase activity ubiquitin protein ligase binding monosaccharide binding protein binding isomerase activity

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