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Invitrogen
GPI Polyclonal Antibody
FIGURE: 1 / 9
GPI Antibody (PA5-29665) in ICC/IF
Immunofluorescence analysis of GPI was performed using 70% confluent log phase HeLa cells. The cells were fixed with ice cold acetone for 5 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GPI Polyclonal Antibody (Product # PA5-29665) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (... View More
Product Details
PA5-29665
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 2 and 287 of Human GPI
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547140
Product Specific Information
Recommended positive controls: H1299, Raji.
Predicted reactivity: Rat (89%), Xenopus laevis (81%), Pig (92%), Rabbit (92%), Chicken (86%), Chimpanzee (99%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AMF; Autocrine motility factor; glucose phosphate isomerase; Glucose-6-phosphate isomerase; glucose-6-phosphate isomerase {ECO:0000250|UniProtKB:P06745}; GPI; GPI {ECO:0000250|UniProtKB:P06745}; gpi {ECO:0000312|RGD:2727}; hexose monophosphate isomerase; hexosephosphate isomerase; Neuroleukin; neuroleukin {ECO:0000250|UniProtKB:P06745}; NLK; NLK {ECO:0000250|UniProtKB:P06745}; oxoisomerase; PGI; PGI {ECO:0000250|UniProtKB:P06745}; PHI; PHI {ECO:0000250|UniProtKB:P06745}; Phosphoglucose isomerase; phosphoglucose isomerase {ECO:0000250|UniProtKB:P06745}; phosphohexomutase; Phosphohexose isomerase; phosphohexose isomerase {ECO:0000250|UniProtKB:P06745}; phosphosaccharomutase; SA-36; sperm antigen; Sperm antigen 36; sperm antigen-36
Gene Aliases: AMF; GNPI; GPI; Gpi1; NLK; PGI; PHI; SA-36; SA36
UniProt ID: (Human) P06744, (Rat) Q6P6V0
Entrez Gene ID: (Human) 2821, (Rat) 292804
angiogenesis
in utero embryonic development
mesoderm formation
carbohydrate metabolic process
gluconeogenesis
humoral immune response
hemostasis
learning or memory
methylglyoxal biosynthetic process
erythrocyte homeostasis
glucose homeostasis
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of neuron apoptotic process
aldehyde catabolic process
glucose 6-phosphate metabolic process
canonical glycolysis
glycolytic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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