Invitrogen

GPI Polyclonal Antibody

GPI Antibody in Immunocytochemistry (ICC/IF)

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GPI Antibody (PA5-29665) in ICC/IF

Immunofluorescence analysis of GPI was performed using 70% confluent log phase HeLa cells. The cells were fixed with ice cold acetone for 5 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GPI Polyclonal Antibody (Product # PA5-29665) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (... View More

GPI Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GPI Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with GPI siRNA and reduction of signal was observed in Western Blot using GPI Polyclonal Antibody (Product # PA5-29665). {KD}

产品信息

PA5-29665

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 2 and 287 of Human GPI

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547140

产品详细信息

Recommended positive controls: H1299, Raji.

Predicted reactivity: Rat (89%), Xenopus laevis (81%), Pig (92%), Rabbit (92%), Chicken (86%), Chimpanzee (99%), Bovine (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness.

Nature cell biology

Thomas GE,Egan G,García-Prat L,Botham A,Voisin V,Patel PS,Hoff FW,Chin J,Nachmias B,Kaufmann KB,Khan DH,Hurren R,Wang X,Gronda M,MacLean N,O'Brien C,Singh RP,Jones CL,Harding SM,Raught B,Arruda A,Minden MD,Bader GD,Hakem R,Kornblau S,Dick JE,Schimmer AD

Published figure using GPI polyclonal antibody (Product # PA5-29665) in Western Blot

Wed Jun 01 00:00:00 UTC 2022

生物信息学

蛋白别名: AMF; Autocrine motility factor; glucose phosphate isomerase; Glucose-6-phosphate isomerase; glucose-6-phosphate isomerase {ECO:0000250|UniProtKB:P06745}; GPI; GPI {ECO:0000250|UniProtKB:P06745}; gpi {ECO:0000312|RGD:2727}; hexose monophosphate isomerase; hexosephosphate isomerase; Neuroleukin; neuroleukin {ECO:0000250|UniProtKB:P06745}; NLK; NLK {ECO:0000250|UniProtKB:P06745}; oxoisomerase; PGI; PGI {ECO:0000250|UniProtKB:P06745}; PHI; PHI {ECO:0000250|UniProtKB:P06745}; Phosphoglucose isomerase; phosphoglucose isomerase {ECO:0000250|UniProtKB:P06745}; phosphohexomutase; Phosphohexose isomerase; phosphohexose isomerase {ECO:0000250|UniProtKB:P06745}; phosphosaccharomutase; SA-36; sperm antigen; Sperm antigen 36; sperm antigen-36

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基因别名: AMF; GNPI; GPI; Gpi1; NLK; PGI; PHI; SA-36; SA36

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UniProt ID: (Human) P06744, (Rat) Q6P6V0

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Entrez Gene ID: (Human) 2821, (Rat) 292804

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功能

glucose-6-phosphate isomerase activity cytokine activity growth factor activity intramolecular transferase activity ubiquitin protein ligase binding monosaccharide binding isomerase

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