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Alomone Labs, LTD
GPR120/FFAR4 (extracellular) Polyclonal Antibody, FITC
FIGURE: 1 / 2
GPR120/FFAR4 (extracellular) Antibody (AFR-014-F-50UL) in Flow
Cell surface detection GPR120/FFAR4 in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + Rabbit IgG Isotype Control-FITC. (green) Cells + Anti-GPR120/FFAR4 (extracellular)-FITC Antibody (#AFR-014-F), (2.5 µg).
Product Details
AFR-014-F-50UL
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4, Extracellular, N-terminus
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 15 µL or 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
An EST for GPR120 has been isolated from a human colon cancer library. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Free fatty acid receptor 4; G protein-coupled receptor 120; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; G-protein-coupled receptor GT01; GPCR120; Omega-3 fatty acid receptor 1
Gene Aliases: AI552415; BMIQ10; FFAR4; GPR120; GPR129; GT01; KPG_013; O3FAR1; PGR4
UniProt ID: (Human) Q5NUL3, (Mouse) Q7TMA4, (Rat) Q2AC31
Entrez Gene ID: (Human) 338557, (Mouse) 107221, (Rat) 294075
G-protein coupled receptor signaling pathway
regulation of glucose transport
cellular response to hormone stimulus
negative regulation of apoptotic process
fat cell differentiation
hormone secretion
negative regulation of cytokine secretion
negative regulation of inflammatory response
detection of chemical stimulus involved in sensory perception of taste
positive regulation of ERK1 and ERK2 cascade
response to peptide
signal transduction
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