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GPT Antibody (PA5-96212) in WB
Western blot analysis of GPT in extracts of various cell lines. Samples were incubated with GPT Polyclonal antibody (Product # PA5-96212) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-96212
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_0053001)
偶联物
形式
Liquid
浓度
1.55 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808014
产品详细信息
Immunogen sequence: MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP; Positive Samples: Mouse liver, Mouse lung, Mouse intestine, Rat liver, Rat heart; Cellular Location: Cytoplasm
靶标信息
GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: alanine aminotransferase; Alanine aminotransferase 1; ALT1; Glutamate pyruvate transaminase 1; glutamic pyruvate transaminase; glutamic pyruvic transaminase (alanine aminotrasferase); glutamic pyruvic transaminase 1, soluble; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; glutamic-alanine transaminase 1; glutamic-pyruvate transaminase (alanine aminotransferase); GPT 1
基因别名: 1300007J06Rik; 2310022B03Rik; AAT1; ALT; ALT1; GPT; Gpt-1; GPT1
UniProt ID: (Human) P24298, (Rat) P25409, (Mouse) Q8QZR5
Entrez Gene ID: (Human) 2875, (Rat) 81670, (Mouse) 76282
