Invitrogen

GRASP65 Polyclonal Antibody

GRASP65 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

GRASP65 Antibody (PA3-910) in ICC/IF

Immunofluorescent analysis of Grasp65 (green) in HeLa cells either left untreated or treated 5 µg/mL Brefeldin A for 4 hours. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Grasp65 rabbit polyclonal antibody (Product # PA3-910) at a dilution of 1:200 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibo... View More

GRASP65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

PA3-910

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:500

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

1:200

产品规格

种属反应

Mouse, Rat, Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

GST fusion protein containing C-terminal residues (202-447) of rat Grasp65.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

whole serum

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2113207

产品详细信息

PA3-910 detects Grasp65 from human, mouse, and rat samples.

PA3-910 has been successfully used in Western blot, IHC (paraffin), immunofluorescence, immunocytochemistry and immunoprecipitation procedures.

The PA3-910 immunogen is a GST fustion protein containing the C-terminal residues (202-447) of rat Grasp65.

靶标信息

Grasp65 is a structural protein of the golgi apparatus. It has been implicated in the stacking of golgi cisternae. Grasp65 is shown to have the properties to bind surfaces together in a mitotically regulated matter.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lack of N-glycosylation increases amyloidogenic processing of the amyloid precursor protein.

Glycobiology

Lin T,van Husen LS,Yu Y,Tjernberg LO,Schedin-Weiss S

PA3-910 was used in Immunocytochemistry-immunofluorescence to suggest that N-glycans have a major impact on trafficking and processing of APP and could play an important role in the development of AD.

Mon May 23 00:00:00 UTC 2022

Lack of N-glycosylation increases amyloidogenic processing of the amyloid precursor protein.

Glycobiology

Lin T,van Husen LS,Yu Y,Tjernberg LO,Schedin-Weiss S

Mon May 23 00:00:00 UTC 2022

Biogenesis of JC polyomavirus associated extracellular vesicles.

Journal of extracellular biology

Morris-Love J,O'Hara BA,Gee GV,Dugan AS,O'Rourke RS,Armstead BE,Assetta B,Haley SA,Atwood WJ

Published figure using GRASP65 polyclonal antibody (Product # PA3-910) in Western Blot

Sun May 01 00:00:00 UTC 2022

Dynamin Inhibitors Prevent the Establishment of the Cytomegalovirus Assembly Compartment in the Early Phase of Infection.

Life (Basel, Switzerland)

Štimac I,Jug Vučko N,Blagojević Zagorac G,Marcelić M,Mahmutefendić Lučin H,Lučin P

PA3-910 was used in Immunocytochemistry to analyse the effect of Dynasore on the Golgi reorganization sequence into the outer preAC.

Wed Aug 25 00:00:00 UTC 2021

Nanofibrous Tubular Three-Dimensional Platform for Single Dental Pulp Stem Cell Polarization.

ACS applied materials & interfaces

Chang B,Ma C,Liu X

PA3-910 was used in Immunocytochemistry to expand the in-depth understanding of DPSC polarization.

Wed Dec 09 00:00:00 UTC 2020

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

PA3-910 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 UTC 2020

Serotonin 5-HT_2C Receptor Cys23Ser Single Nucleotide Polymorphism Associates with Receptor Function and Localization In Vitro.

Scientific reports

Land MA,Chapman HL,Davis-Reyes BD,Felsing DE,Allen JA,Moeller FG,Elferink LA,Cunningham KA,Anastasio NC

PA3-910 was used in Immunocytochemistry to show a single nucleotide polymorphism of human serotonin 5-HT2C receptor causing the Cys23Ser mutation results in a distinct pharmacological and subcellular localization profile of the receptor versus the wild-type.

Wed Nov 13 00:00:00 UTC 2019

Autophagy-lysosome pathway alterations and alpha-synuclein up-regulation in the subtype of neuronal ceroid lipofuscinosis, CLN5 disease.

Scientific reports

Adams J,Feuerborn M,Molina JA,Wilden AR,Adhikari B,Budden T,Lee SY

PA3-910 was used in Immunohistochemistry to examine whether autophagy is altered in CLN5 disease.

Thu Jan 17 00:00:00 UTC 2019

生物信息学

蛋白别名: Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; golgi reassembly stacking protein 1, 65kDa; Golgi reassembly-stacking protein 1; Golgi reassembly-stacking protein of 65 kDa; GOLPH5; GRASP65

查看更多收起

基因别名: 5430411C10Rik; GOLPH5; GORASP1; GRASP65; P65

查看更多收起

UniProt ID: (Human) Q9BQQ3, (Mouse) Q91X51

查看更多收起

Entrez Gene ID: (Human) 64689, (Rat) 56082, (Mouse) 74498

查看更多收起

功能

protein binding molecular_function

参与通路

Golgi organization protein transport negative regulation of dendrite morphogenesis protein N-linked glycosylation ER to Golgi vesicle-mediated transport COPII vesicle coating transport