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GRK1 Antibody (MA1-721) in ICC/IF
Immunofluorescent analysis of Rhodopsin Kinase 1a/b (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) (Product # MA1-721) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. ... View More
产品信息
MA1-721
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse
已发表种属
Dog,
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
D11
抗原
Full length human GRK1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2263842
产品详细信息
MA1-721 detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.
MA1-721 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation experiments. By Western blot, this antibody detects an ~60 kDa protein representing GRK 1a/b. MA1-721 detects both in vitro expressed splice variants by Western blot but was not able to detect significant amounts of GRK1b from human retina. Immunofluorescence staining of GRK1a/b in bovine retina with MA1-721 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.
The MA1-721 immunogen is full length human GRK1a. This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.
靶标信息
GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro. It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: G protein-coupled receptor kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1a; rhodopsin kinase; Rhodopsin kinase GRK1; RK
基因别名: GPRK1; GRK1; RHOK; RK
UniProt ID: (Human) Q15835, (Bovine) P28327
Entrez Gene ID: (Human) 6011, (Bovine) 281457, (Mouse) 24013
protein kinase activity
G-protein coupled receptor kinase activity
ATP binding
rhodopsin kinase activity
protein binding
nucleotide binding
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
visual perception
regulation of G-protein coupled receptor protein signaling pathway
rhodopsin mediated signaling pathway
regulation of rhodopsin mediated signaling pathway
protein autophosphorylation
signal transduction
protein phosphorylation
photoreceptor cell morphogenesis
response to light stimulus
phosphorylation
termination of G-protein coupled receptor signaling pathway
positive regulation of phosphorylation
negative regulation of apoptotic process
response to stimulus
post-embryonic retina morphogenesis in camera-type eye
