Invitrogen

GRK1 Monoclonal Antibody (D11)

2 Published Figures

6 References

View all (19) GRK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (2)

GRK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

GRK1 Antibody (MA1-721) in ICC/IF

Immunofluorescent analysis of Rhodopsin Kinase 1a/b (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) (Product # MA1-721) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. ... View More

GRK1 Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-721

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Published species

Dog, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

D11

Immunogen

Full length human GRK1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2263842

Product Specific Information

MA1-721 detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.

MA1-721 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation experiments. By Western blot, this antibody detects an ~60 kDa protein representing GRK 1a/b. MA1-721 detects both in vitro expressed splice variants by Western blot but was not able to detect significant amounts of GRK1b from human retina. Immunofluorescence staining of GRK1a/b in bovine retina with MA1-721 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.

The MA1-721 immunogen is full length human GRK1a. This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

Target Information

GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro. It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP.

The Journal of biological chemistry

Chrispell JD,Xiong Y,Weiss ER

MA1-721 was used in Immunohistochemistry to provide a better understanding of the function of Grk phosphorylation relative to cone adaptation and recovery.

Thu Dec 01 00:00:00 UTC 2022

Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP.

The Journal of biological chemistry

Chrispell JD,Xiong Y,Weiss ER

MA1-721 was used in Immunohistochemistry to provide a better understanding of the function of Grk phosphorylation relative to cone adaptation and recovery.

Thu Dec 01 00:00:00 UTC 2022

Acute and Protracted Cell Death in Light-Induced Retinal Degeneration in the Canine Model of Rhodopsin Autosomal Dominant Retinitis Pigmentosa.

Investigative ophthalmology & visual science

Sudharsan R,Simone KM,Anderson NP,Aguirre GD,Beltran WA

MA1-721 was used in Immunohistochemistry to characterise outcome measures of acute and continuing retinal degeneration in the rhodopsin T4R dog model following light exposure.

Sun Jan 01 00:00:00 UTC 2017

GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tsang SH,Woodruff ML,Chen CK,Yamashita CY,Cilluffo MC,Rao AL,Farber DB,Fain GL

MA1-721 was used in western blot to study the effect of the cGMP-phosphodiesterase gamma subunit on photoreceptor light response termination.

Wed Apr 26 00:00:00 UTC 2006

GRK1-dependent phosphorylation of S and M opsins and their binding to cone arrestin during cone phototransduction in the mouse retina.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhu X,Brown B,Li A,Mears AJ,Swaroop A,Craft CM

MA1-721 was used in immunohistochemistry to investigate the role of GRK1-dependent phosphorylation of cone opsins during the murine cone phototransduction.

Wed Jul 09 00:00:00 UTC 2003

A short, highly active photoreceptor-specific enhancer/promoter region upstream of the human rhodopsin kinase gene.

Investigative ophthalmology & visual science

Young JE,Vogt T,Gross KW,Khani SC

MA1-721 was used in immunohistochemistry to define a photoreceptor-specific enhancer-promoter upstream of the Rk gene.

Mon Sep 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: G protein-coupled receptor kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1a; rhodopsin kinase; Rhodopsin kinase GRK1; RK

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Gene Aliases: GPRK1; GRK1; RHOK; RK

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UniProt ID: (Human) Q15835, (Bovine) P28327

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Entrez Gene ID: (Human) 6011, (Bovine) 281457, (Mouse) 24013

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Function(s)

protein kinase activity G-protein coupled receptor kinase activity ATP binding rhodopsin kinase activity protein binding nucleotide binding protein serine/threonine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

visual perception regulation of G-protein coupled receptor protein signaling pathway rhodopsin mediated signaling pathway regulation of rhodopsin mediated signaling pathway protein autophosphorylation signal transduction protein phosphorylation photoreceptor cell morphogenesis response to light stimulus phosphorylation termination of G-protein coupled receptor signaling pathway positive regulation of phosphorylation negative regulation of apoptotic process response to stimulus post-embryonic retina morphogenesis in camera-type eye

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GRK1 Monoclonal Antibody (D11)

GRK1 Antibody (MA1-721) in ICC/IF

Product Details

MA1-721

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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